| Title |
Protein-Glass Surface Interactions and Ion Desalting in Electrospray Ionization with Submicron Emitters
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, October 2017
|
| DOI | 10.1007/s13361-017-1825-6 |
| Pubmed ID | |
| Authors |
Zije Xia, Evan R. Williams |
| Abstract |
Theta glass electrospray emitters can rapidly mix solutions to investigate fast reactions that occur as quickly as 1 μs, but emitters with submicron tips have the unusual properties of desalting protein ions and affecting the observed abundances of some proteins as a result of protein-surface interactions. The role of protein physical properties on ion signal was investigated using 1.7 ± 0.1 μm and 269 ± 7 nm emitters and 100 mM aqueous ammonium acetate or ammonium bicarbonate solutions. Protein ion desalting occurs for both positive and negative ions. The signal of a mixture of proteins with the 269 nm tips is time-dependent and the order in which ions of each protein is observed is related to the expected strengths of the protein-surface interactions. These results indicate that it is not just the high surface-to-volume ratio that plays a role in protein adsorption and reduction or absence of initial ion signal, but the small diffusion distance and extremely low flow rates of the smaller emitters can lead to complete adsorption of some proteins and loss of signal until the adsorption sites are filled and the zeta potential is significantly reduced. After about 30 min, signals for a protein mixture from the two different size capillaries are similar. These results show the advantages of submicron emitters but also indicate that surface effects must be taken into account in experiments using such small tips or that coating the emitter surface to prevent adsorption should be considered. Graphical Abstract. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| United Kingdom | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 32% |
| Student > Master | 6 | 14% |
| Student > Bachelor | 5 | 11% |
| Professor | 3 | 7% |
| Researcher | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 11 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 24 | 55% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Physics and Astronomy | 1 | 2% |
| Agricultural and Biological Sciences | 1 | 2% |
| Medicine and Dentistry | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 11 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2017.
All research outputs
#17,292,294
of 25,382,440 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#2,724
of 3,835 outputs
Outputs of similar age
#213,524
of 334,091 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#27
of 59 outputs
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