| Title |
Antimicrobial and Antioxidant Activities of Clove Essential Oil and Eugenyl Acetate Produced by Enzymatic Esterification
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, August 2014
|
| DOI | 10.1007/s12010-014-1113-x |
| Pubmed ID | |
| Authors |
Adriana B. Vanin, Tainara Orlando, Suelen P. Piazza, Bruna M. S. Puton, Rogério L. Cansian, Debora Oliveira, Natalia Paroul |
| Abstract |
This work reports the maximization of eugenyl acetate production by esterification of essential oil of clove in a solvent-free system using Novozym 435 as catalyst. The antimicrobial and antioxidant activities of clove essential oil and eugenyl acetate produced were determined. The conditions that maximized eugenyl acetate production were 60 °C, essential oil of clove to acetic anhydride ratio of 1:5, 150 rpm, and 10 wt% of enzyme, with a conversion of 99.87 %. A kinetic study was performed to assess the influence of substrates' molar ratio, enzyme concentration, and temperature on product yield. Results show that an excess of anhydride, enzyme concentration of 5.5 wt%, 50 °C, and essential oil of clove to acetic anhydride ratio of 1:5 afforded nearly a complete conversion after 2 h of reaction. Comparing the antibacterial activity of the essential oil of clove before and after esterification, we observed a decrease in the antimicrobial activity of eugenyl acetate, particularly with regard to minimum inhibitory concentration (MIC). Both eugenyl acetate and clove essential oil were most effective to the gram-negative than gram-positive bacteria group. The results showed a high antioxidant potential for essential oil before and particularly after the esterification reaction thus becoming an option for the formulation of new antioxidant products. |
Mendeley readers
The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 1 | <1% |
| France | 1 | <1% |
| Unknown | 122 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 23 | 19% |
| Student > Master | 21 | 17% |
| Researcher | 8 | 6% |
| Student > Ph. D. Student | 8 | 6% |
| Professor | 8 | 6% |
| Other | 16 | 13% |
| Unknown | 40 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 18 | 15% |
| Agricultural and Biological Sciences | 17 | 14% |
| Engineering | 12 | 10% |
| Biochemistry, Genetics and Molecular Biology | 5 | 4% |
| Chemical Engineering | 5 | 4% |
| Other | 18 | 15% |
| Unknown | 49 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 February 2015.
All research outputs
#18,379,655
of 22,765,347 outputs
Outputs from Applied Biochemistry and Biotechnology
#1,771
of 2,499 outputs
Outputs of similar age
#164,415
of 230,513 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#28
of 60 outputs
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So far Altmetric has tracked 2,499 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
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We're also able to compare this research output to 60 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.