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X Demographics
Mendeley readers
Attention Score in Context
| Title |
RNA polymerase II stalling at pre-mRNA splice sites is enforced by ubiquitination of the catalytic subunit
|
|---|---|
| Published in |
eLife, October 2017
|
| DOI | 10.7554/elife.27082 |
| Pubmed ID | |
| Authors |
Laura Milligan, Camille Sayou, Alex Tuck, Tatsiana Auchynnikava, Jane EA Reid, Ross Alexander, Flavia de Lima Alves, Robin Allshire, Christos Spanos, Juri Rappsilber, Jean D Beggs, Grzegorz Kudla, David Tollervey |
| Abstract |
Numerous links exist between co-transcriptional RNA processing and the transcribing RNAPII. In particular, pre-mRNA splicing was reported to be associated with slowed RNAPII elongation. Here we identify a site of ubiquitination (K1246) in the catalytic subunit of RNAPII close to the DNA entry path. Ubiquitination was increased in the absence of the Bre5-Ubp3 ubiquitin protease complex. Bre5 binds RNA in vivo, with a preference for exon 2 regions of intron-containing pre-mRNAs and poly(A) proximal sites. Ubiquitinated RNAPII showed similar enrichment. The absence of Bre5 led to impaired splicing and defects in RNAPII elongation in vivo on a splicing reporter construct. Strains expressing RNAPII with a K1246R mutation showed reduced cotranscriptional splicing. We propose that ubiquinitation of RNAPII is induced by RNA processing events and linked to transcriptional pausing, which is released by Bre5-Ubp3 associated with the nascent transcript. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 25% |
| Austria | 1 | 13% |
| Australia | 1 | 13% |
| Germany | 1 | 13% |
| Unknown | 3 | 38% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 4 | 50% |
| Members of the public | 4 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 55 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 29% |
| Researcher | 6 | 11% |
| Student > Bachelor | 5 | 9% |
| Professor > Associate Professor | 5 | 9% |
| Student > Doctoral Student | 3 | 5% |
| Other | 7 | 13% |
| Unknown | 13 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 23 | 42% |
| Agricultural and Biological Sciences | 12 | 22% |
| Neuroscience | 2 | 4% |
| Chemistry | 2 | 4% |
| Unspecified | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 13 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 August 2021.
All research outputs
#6,865,426
of 23,005,189 outputs
Outputs from eLife
#10,319
of 13,940 outputs
Outputs of similar age
#111,292
of 325,897 outputs
Outputs of similar age from eLife
#277
of 403 outputs
Altmetric has tracked 23,005,189 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 13,940 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.9. This one is in the 25th percentile – i.e., 25% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 325,897 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 403 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.