| Title |
Cloning and upscale production of monoamine oxidase N (MAO-N D5) by Pichia pastoris
|
|---|---|
| Published in |
Biotechnology Techniques, October 2017
|
| DOI | 10.1007/s10529-017-2450-y |
| Pubmed ID | |
| Authors |
Kristína Markošová, Andrea Camattari, Michal Rosenberg, Anton Glieder, Nicholas J. Turner, Martin Rebroš |
| Abstract |
To clone monoamine oxidase N, that catalyses the selective oxidative deamination or deracemisation of amines into imines, in Pichia pastoris and prove the importance of choosing the proper expression system for its recombinant production. Monoamine oxidase, originating from Aspergillus niger and subjected to directed evolution (MAO-N D5), was cloned and expressed in Pichia pastoris CBS7435 Mut(S) strain for the first time. Various transformants were screened at microscale level. The production of the clone expressing the most active enzyme was scaled-up to a 1.5 l fermenter and preparation of MAO-N D5 as a crude enzyme extract was optimised. The obstacles in the production of the enzyme in both expression systems, Escherichia coli and P. pastoris, are discussed and demonstrated. There was an improvement in specific productivity, which was 83 times higher in P. pastoris, clearly proving the importance of choosing the right expression host system for the specific enzymes. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 16% |
| Student > Ph. D. Student | 4 | 16% |
| Student > Master | 3 | 12% |
| Student > Bachelor | 2 | 8% |
| Other | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 9 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 32% |
| Chemistry | 3 | 12% |
| Agricultural and Biological Sciences | 2 | 8% |
| Unknown | 12 | 48% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2017.
All research outputs
#19,951,180
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Outputs from Biotechnology Techniques
#2,303
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#243,508
of 333,631 outputs
Outputs of similar age from Biotechnology Techniques
#12
of 20 outputs
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