PCR Protocols

Table of Contents

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   Book Overview
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   Chapter -896032441 Polymerase Chain Reaction: Basic Protocols
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   Chapter -896032411 Selection of Primers for Polymerase Chain Reaction
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   Chapter -896032401 Direct Radioactive Labeling of Polymerase Chain Reaction Products
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   Chapter -896032397 Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays
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   Chapter -896032391 Nonradioactive Labeling of Polymerase Chain Reaction Products
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   Chapter -896032379 Quantitation and Purification of Polymerase Chain Reaction Products by High-Performance Liquid Chromatography
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   Chapter -896032367 Use of Polymerase Chain Reaction for Screening Transgenic Mice
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   Chapter -896032361 Polymerase Chain Reaction Analysis of DNA from Paraffin-Embedded Tissue
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    Chapter -896032353 The Use of Polymerase Chain Reaction for Chromosome Assignment
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    Chapter -896032347 Mapping MHC Class II Genes and Disease-Susceptibility: Use of Polymerase Chain Reaction and Dot Hybridization for Human Leukocyte Antigen Allele Typing
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    Chapter -896032329 The Use of the Polymerase Chain Reaction and the Detection of Amplified Products
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    Chapter -896032313 Determination of Loss of Heterozygosity Using Polymerase Chain Reaction
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    Chapter -896032305 Direct Sequencing of Polymerase Chain Reaction Products
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    Chapter -896032299 Manual and Automated Direct Sequencing of Product Generated by the Polymerase Chain Reaction
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    Chapter -896032289 Genomic Footprinting by Ligation Mediated Polymerase Chain Reaction
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    Chapter -896032273 RNA Template-Specific Polymerase Chain Reaction (RS-PCR): A Modification of RNA-PCR that Dramatically Reduces the Frequency of False Positives
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    Chapter -896032265 Quantitative Measurement of Relative Gene Expression in Human Tumors
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    Chapter -896032253 Identification of Alternatively Spliced mRNAs and Localization of 5' Ends by Polymerase Chain Reaction Amplification
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    Chapter -896032243 Utilization of Polymerase Chain Reaction for Clonal Analysis of Gene Expression
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    Chapter -896032237 Sequencing DNA Amplified Directly from a Bacterial Colony
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    Chapter -896032231 Use of Polymerase Chain Reaction to Screen Phage Libraries
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    Chapter -896032225 Molecular Cloning of Polymerase Chain Reaction Fragments with Cohesive Ends
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    Chapter -896032213 Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products
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    Chapter -896032201 Use of Polymerase Chain Reaction for Making Recombinant Constructs
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    Chapter -896032191 In Vitro Recombination and Mutagenesis of DNA: SOEing Together Tailor-Made Genes
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    Chapter -896032179 Use of Polymerase Chain Reaction for the Rapid Construction of Synthetic Genes
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    Chapter -896032173 Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis
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    Chapter -896032165 Site-Directed Mutagenesis by Double Polymerase Chain Reaction: Megaprimer Method
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    Chapter -896032155 Generation of a Polymerase Chain Reaction Renewable Source of Subtractive cDNA
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    Chapter -896032137 PCR-Based Full-Length cDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-Pair Strategy
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    Chapter -896032125 Use of Degenerate Oligonucleotide Primers and the Polymerase Chain Reaction to Clone Gene Family Members
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    Chapter -896032103 Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking
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    Chapter -896032093 cDNA Cloning by Inverse Polymerase Chain Reaction
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    Chapter -896032085 Amplification of Gene Ends from Gene Libraries by Polymerase Chain Reaction with Single-Sided Specificity
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    Chapter -896032077 Anchoring a Defined Sequence to the 5' Ends of mRNAs: The Bolt to Clone Rare Full Length mRNAs and Generate cDNA Libraries from a Few Cells