| Chapter title |
Cell Cycle Analysis by Mass Cytometry
|
|---|---|
| Chapter number | 8 |
| Book title |
Cellular Quiescence
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7371-2_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7370-5, 978-1-4939-7371-2
|
| Authors |
Gregory K. Behbehani |
| Abstract |
The regulated progression of cells through the cell cycle during proliferation is a critical factor in tumor progression, anti-neoplastic therapy response, immune system regulation, and developmental biology. While flow cytometric measurement of cell cycle progression is well established, mass cytometry assays allow the cell cycle to be measured along with up to 39 other antigens enabling characterization of the complex interactions between the cell cycle and a wide variety of cellular processes. This method describes the use of mass cytometry for the analysis of cell cycle state for cells from three different sources: in vitro cultured cell lines, ex vivo human blood or bone marrow, and in vivo labeling and ex vivo analysis of murine tissues. The method utilizes incorporation of 5-Iodo-2'-deoxyuridine (IdU), combined with measurement of phosphorylated retinoblastoma protein (pRb), cyclin B1, and phosphorylated histone H3 (p-HH3). These measurements can be integrated into a gating strategy that allows for clear separation of all five phases of the cell cycle. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 50% |
| United Kingdom | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 41 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 27% |
| Student > Master | 5 | 12% |
| Researcher | 5 | 12% |
| Student > Bachelor | 4 | 10% |
| Professor | 3 | 7% |
| Other | 2 | 5% |
| Unknown | 11 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 22% |
| Agricultural and Biological Sciences | 5 | 12% |
| Immunology and Microbiology | 4 | 10% |
| Medicine and Dentistry | 4 | 10% |
| Engineering | 2 | 5% |
| Other | 6 | 15% |
| Unknown | 11 | 27% |