Chapter title |
MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans
|
---|---|
Chapter number | 5 |
Book title |
Argonaute Proteins
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7339-2_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7338-5, 978-1-4939-7339-2
|
Authors |
Andachi, Yoshiki, Kohara, Yuji, Yoshiki Andachi, Yuji Kohara |
Abstract |
MicroRNAs (miRNAs) loaded on argonaute proteins guide RNA-induced silencing complexes to target mRNAs. An excellent method to decipher the spatiotemporal expression patterns of miRNAs is whole-mount in situ hybridization (WISH), which has been successfully used in vertebrate embryos but still remains unavailable for many animal species. Here, we describe a WISH method for miRNA detection in Caenorhabditis elegans at both embryonic and post-embryonic stages. Strategies devised for detection include fixation of animals with carbodiimide at a high temperature and subsequent partial digestion of the fixed animals with an extremely high concentration of proteinase. WISH signals are visualized by staining with a chromogenic substrate or a fluorescent dye. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 33% |
Researcher | 1 | 17% |
Unknown | 3 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 50% |
Unknown | 3 | 50% |