Chapter title |
Generation of Mammalian Host-Adapted Borrelia burgdorferi by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats
|
---|---|
Chapter number | 3 |
Book title |
Borrelia burgdorferi
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7383-5_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7382-8, 978-1-4939-7383-5
|
Authors |
Melissa J. Caimano |
Abstract |
The transmission, survival, and virulence of Borrelia burgdorferi depend upon the spirochete's ability to modulate its transcriptome as it cycles between its arthropod vector and reservoir host. This complex adaptive process is collectively referred to as "host-adaptation." The paucibacillary nature of borrelial infections precludes the detailed analysis of host adaptation within infected mammalian tissues. To circumvent this limitation, we (J Clin Invest 101:2240-2250, 1998) developed a model system whereby spirochetes are cultivated within dialysis membrane chambers (DMCs) surgically implanted within the peritoneal cavity of a rat. Spirochetes within DMCs are exposed to many, if not all, of the environmental signals and physiological cues required for mammalian host adaptation but are protected from clearance by the host's immune system. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Canada | 1 | 33% |
United States | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 2 | 14% |
Student > Ph. D. Student | 2 | 14% |
Researcher | 2 | 14% |
Other | 1 | 7% |
Professor | 1 | 7% |
Other | 3 | 21% |
Unknown | 3 | 21% |
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Medicine and Dentistry | 2 | 14% |
Immunology and Microbiology | 2 | 14% |
Nursing and Health Professions | 1 | 7% |
Unspecified | 1 | 7% |
Other | 2 | 14% |
Unknown | 3 | 21% |