| Title |
Enantioselective separation and determination of miconazole in rat plasma by chiral LC–MS/MS: application in a stereoselective pharmacokinetic study
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, August 2017
|
| DOI | 10.1007/s00216-017-0551-z |
| Pubmed ID | |
| Authors |
Yueying Du, Linda Luo, Shuo Sun, Zhen Jiang, Xingjie Guo |
| Abstract |
Miconazole has one chiral center, and consists of two enantiomers. In this study, a novel chiral liquid chromatography-tandem mass spectrometry method was developed for enantioselective separation and determination of miconazole in rat plasma. For the first time, the enantioselective pharmacokinetics of miconazole was investigated by the current method. Firstly, attempts were made to separate the enantiomers in reversed-phase mode with a mobile phase that was mass spectrometry compatible. Baseline separation was achieved on a Chiralpak IC column with a mobile phase composed of acetonitrile and aqueous ammonium hydrogen carbonate (5 mM; 80:20, v/v). Data were acquired in multiple reaction monitoring mode with positive electrospray ionization by triple-quadrupole mass spectrometry. Then, overall method validation regarding the linearity, accuracy, precision, extraction recovery, matrix effect, and stability of each enantiomer was performed, and acceptable results were obtained for all of these. Finally, the method developed was applied in an enantioselective pharmacokinetic study of miconazole enantiomers in rats after oral administration of racemic miconazole at doses of 5 and 10 mg/kg. The results demonstrated that (-)-(R)-miconazole had a higher concentration than (+)-(S)-miconazole in plasma, with a ratio of 1.3-1.7 for both doses. This is the first experimental evidence of enantioselective behavior of miconazole in vivo, and provides a reference for clinical practice and encourages further research into miconazole enantioselective metabolism and drug interactions. Graphical Abstract A stereoselective pharmacokinetic study of the miconazole enantiomers was investigated using a novel chiral liquid chromatography-tandem mass spectrometry method. Baseline separation was achieved on Chiralpak IC column, and Chiralcel OJ column was used to collect single enantiomer. A significant difference between the two enantiomers was observed in view of the plasma concentration. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 75% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 13% |
| Professor > Associate Professor | 2 | 13% |
| Unspecified | 1 | 6% |
| Professor | 1 | 6% |
| Student > Doctoral Student | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 7 | 44% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 19% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 13% |
| Unspecified | 1 | 6% |
| Materials Science | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 8 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2017.
All research outputs
#15,989,045
of 25,382,440 outputs
Outputs from Analytical & Bioanalytical Chemistry
#4,917
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Outputs of similar age
#182,822
of 323,804 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#47
of 172 outputs
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