| Title |
The hypertrehalosemic neuropeptides of cicadas are structural isomers—evidence by ion mobility mass spectrometry
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, August 2017
|
| DOI | 10.1007/s00216-017-0583-4 |
| Pubmed ID | |
| Authors |
Simone König, Heather Marco, Gerd Gäde |
| Abstract |
It has been known for more than 20 years that the neurosecretory glands of the cicadas, the corpora cardiaca, synthesize two isobaric peptides with hypertrehalosemic activity. Both decapeptides have exactly the same amino acid sequence (pGlu-Val-Asn-Phe-Ser-Pro-Ser-Trp-Gly-Asn-NH2) and mass but differ in their retention time in reversed-phase liquid chromatography. A synthetic peptide with the same sequence elutes together with the second more hydrophobic peptide peak of the natural cicada extract. It is not clear what modification is causing the described observations. Therefore, in the current study, ion mobility separation in conjunction with high-resolution mass spectrometry was used to investigate this phenomenon as it was sensitive to changes in conformation. It detected different drift times in buffer gas for both the intact peptides and some of their fragment ions. Based on the ion mobility and fragment ion intensity of the corresponding ions, it is concluded that the region Pro(6)-Ser(7)-Trp(8) contains a structural feature differing from the L-amino acids present in the known peptide. Whether the conformer is the result of racemization or other biochemical processes needs to be further investigated. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 23% |
| Student > Ph. D. Student | 2 | 15% |
| Librarian | 1 | 8% |
| Other | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Other | 3 | 23% |
| Unknown | 2 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 5 | 38% |
| Unspecified | 1 | 8% |
| Arts and Humanities | 1 | 8% |
| Agricultural and Biological Sciences | 1 | 8% |
| Neuroscience | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 4 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 November 2017.
All research outputs
#19,951,180
of 25,382,440 outputs
Outputs from Analytical & Bioanalytical Chemistry
#6,061
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Outputs of similar age
#236,307
of 323,804 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#64
of 172 outputs
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