| Title |
Molecular interaction of α-synuclein with tubulin influences on the polymerization of microtubule in vitro and structure of microtubule in cells
|
|---|---|
| Published in |
Molecular Biology Reports, October 2009
|
| DOI | 10.1007/s11033-009-9899-2 |
| Pubmed ID | |
| Authors |
R. M. Zhou, Y. X. Huang, X. L. Li, C. Chen, Q. Shi, G. R. Wang, C. Tian, Z. Y. Wang, Y. Y. Jing, C. Gao, X. P. Dong |
| Abstract |
Microtubule dynamics is essential for many vital cellular processes such as in intracellular transport, metabolism, and cell division. Evidences demonstrate that α-synuclein may associate with microtubular cytoskeleton and its major component, tubulin. In the present study, the molecular interaction between α-synuclein and tubulin was confirmed by GST pull-down assay and co-immunoprecipitation. The interacting regions within α-synuclein with tubulin were mapped at the residues 60-100 of α-synuclein that is critical for the binding activity with tubulin. Microtubule assembly assays and sedimentation tests demonstrated that α-synuclein influenced the polymerization of tubulin in vitro, revealing an interacting region-dependent feature. Confocal microscopy detected that exposures of α-synuclein proteins inhibited microtubule formation in the cultured cells, with a length-dependent phenomenon. Our data highlight a potential role of α-synuclein in regulating the microtubule dynamics in neurons. The association of α-synuclein with tubulin may further provide insight into the biological and pathophysiological function of synuclein. |
Mendeley readers
The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 2 | 2% |
| Unknown | 96 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 21% |
| Student > Master | 12 | 12% |
| Student > Bachelor | 12 | 12% |
| Researcher | 12 | 12% |
| Other | 3 | 3% |
| Other | 10 | 10% |
| Unknown | 28 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 24 | 24% |
| Agricultural and Biological Sciences | 16 | 16% |
| Neuroscience | 14 | 14% |
| Medicine and Dentistry | 6 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 4% |
| Other | 6 | 6% |
| Unknown | 28 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2014.
All research outputs
#15,307,723
of 22,766,595 outputs
Outputs from Molecular Biology Reports
#1,116
of 2,894 outputs
Outputs of similar age
#77,591
of 93,128 outputs
Outputs of similar age from Molecular Biology Reports
#5
of 6 outputs
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So far Altmetric has tracked 2,894 research outputs from this source. They receive a mean Attention Score of 2.2. This one has gotten more attention than average, scoring higher than 50% of its peers.
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