| Title |
Mir-302 cluster exhibits tumor suppressor properties on human unrestricted somatic stem cells
|
|---|---|
| Published in |
Tumor Biology, April 2014
|
| DOI | 10.1007/s13277-014-1844-x |
| Pubmed ID | |
| Authors |
Fatemeh Jamshidi-Adegani, Lida Langroudi, Abbas Shafiee, Abdollah Mohammadi-Sangcheshmeh, Abdolreza Ardeshirylajimi, Mansoureh Barzegar, Keyhan Azadmanesh, Mahmood Naderi, Ehsan Arefian, Masoud Soleimani |
| Abstract |
Many studies have reported that miR-302-367 cluster acts in different ways in various cell types. For instance, this cluster is shown to have a potential role in stemness regulation in embryonic stem cells (ESCs). On the other hand, this cluster inhibits the tumorigenicity of human pluripotent stem cells by coordinated suppression of CDK2 and CDK4/6 cell cycle pathways. Indeed, this cluster has a significant posttranscriptional impact on cell cycle progression. Previous reports have shown the participation of miR-302-367 cluster in cell cycle regulation of hESCs, MCF7, HepG2, and Teta-2 embryonal teratocarcinoma cells, but its effect on unrestricted somatic stem cells (USSCs) as a new source of human somatic stem cells from the umbilical cord blood remains to be elucidated. Therefore, in this study, we aimed to investigate the effect of miR-302-367 cluster on cell proliferation by MTT assay, cell cycle analysis, and colony formation assay. In addition, the expression of candidate cell cycle regulatory performance and tumor suppressor genes was determined. In this study, for the first time, we found that miR-302-367 cluster not only did not reprogram human USSCs into a pluripotent ESC-like state, but also inhibited the proliferation of human USSCs. Moreover, analyzing the cell cycle curve revealed a significant apoptotic phase upon viral introduction of miR-302-367. Our gene expression study revealed the overexpression of candidate genes after transduction of USSCs with miR-302-367 cluster. In conclusion, the controversial role of miR-302-367 in different cell types may provide better understanding for its role in stemness level and its antitumorigenicity potential in different contexts. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 22% |
| Researcher | 5 | 19% |
| Professor > Associate Professor | 4 | 15% |
| Student > Bachelor | 3 | 11% |
| Professor | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 30% |
| Biochemistry, Genetics and Molecular Biology | 6 | 22% |
| Medicine and Dentistry | 3 | 11% |
| Neuroscience | 2 | 7% |
| Nursing and Health Professions | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 5 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2014.
All research outputs
#20,239,689
of 22,766,595 outputs
Outputs from Tumor Biology
#1,834
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Outputs of similar age
#193,158
of 226,097 outputs
Outputs of similar age from Tumor Biology
#50
of 74 outputs
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