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Base pairing between hepatitis C virus RNA and 18S rRNA is required for IRES-dependent translation initiation in vivo

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, October 2014
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  • Good Attention Score compared to outputs of the same age (66th percentile)

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Title
Base pairing between hepatitis C virus RNA and 18S rRNA is required for IRES-dependent translation initiation in vivo
Published in
Proceedings of the National Academy of Sciences of the United States of America, October 2014
DOI 10.1073/pnas.1413472111
Pubmed ID
Authors

Daiki Matsuda, Vincent P. Mauro

Abstract

Degeneracy in eukaryotic translation initiation is evident in the initiation strategies of various viruses. Hepatitis C virus (HCV) provides an exceptional example--translation of the HCV RNA is facilitated by an internal ribosome entry site (IRES) that can autonomously bind a 40S ribosomal subunit and accurately position it at the initiation codon. This binding involves both ribosomal protein and 18S ribosomal RNA (rRNA) interactions. In this study, we evaluate the functional significance of the rRNA interaction and show that HCV IRES activity requires a 3-nt Watson-Crick base-pairing interaction between the apical loop of subdomain IIId in the IRES and helix 26 in 18S rRNA. Mutations of these nucleotides in either RNA dramatically disrupted IRES activity. The activities of the mutated HCV IRESs could be restored by compensatory mutations in the 18S rRNA. The effects of the 18S rRNA mutations appeared to be specific inasmuch as ribosomes containing these mutations did not support translation mediated by the wild-type HCV IRES, but did not block translation mediated by the cap structure or other viral IRESs. The present study provides, to our knowledge, the first functional demonstration of mRNA-rRNA base pairing in mammalian cells. By contrast with other rRNA-binding sites in mRNAs that can enhance translation as independent elements, e.g., the Shine-Dalgarno sequence in prokaryotes, the rRNA-binding site in the HCV IRES functions as an essential component of a more complex interaction.

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X Demographics

The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Croatia 1 2%
Unknown 60 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 26%
Researcher 11 18%
Student > Bachelor 7 11%
Student > Master 5 8%
Professor > Associate Professor 4 6%
Other 6 10%
Unknown 13 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 25 40%
Agricultural and Biological Sciences 18 29%
Immunology and Microbiology 2 3%
Computer Science 1 2%
Environmental Science 1 2%
Other 2 3%
Unknown 13 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 November 2020.
All research outputs
#8,159,315
of 24,625,114 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#64,394
of 101,438 outputs
Outputs of similar age
#85,778
of 261,070 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#685
of 956 outputs
Altmetric has tracked 24,625,114 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 101,438 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 38.8. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 261,070 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 956 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.