| Title |
1-Ethyl-3-methylimidazolium tolerance and intracellular lipid accumulation of 38 oleaginous yeast species
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, October 2017
|
| DOI | 10.1007/s00253-017-8506-z |
| Pubmed ID | |
| Authors |
Irnayuli R. Sitepu, Luis A. Garay, Lauren Enriquez, Russell Fry, John H. Butler, Julian M. Lopez, Atit Kanti, Sarah A. Faulina, Agustinus J. Nugroho, Blake A. Simmons, Steven W. Singer, Christopher W. Simmons, Kyria Boundy-Mills |
| Abstract |
Pretreatment with ionic liquids (IL) such as 1-ethyl-3-methylimidazolium chloride or acetate is an effective method for aiding deconstruction of lignocellulosic biomass; however, the residual IL remaining in hydrolysates can be inhibitory to growth of ethanologenic or oleaginous yeasts that have been examined in the literature. The aim of this study was to identify oleaginous yeasts that are tolerant of the IL [C2C1Im][OAc] and [C2C1Im]Cl using 45 strains belonging to 38 taxonomically diverse species within phyla Ascomycota and Basidiomycota. Yeasts were cultivated in laboratory medium supplemented with 0, 2, or 4% IL in 96-well plates. The eight most tolerant strains were then cultivated in 10-mL media with no IL, 242mM [C2C1Im][OAc], or 242mM [C2C1Im]Cl. The effects of [C2C1Im](+) exposure on cell mass production and lipid accumulation varied at the species and strain level. The acetate salt decreased cell biomass and lipid production more severely than did the chloride ion for six strains. Lipid output was not markedly different (2.1 vs. 2.3 g/L) in Yarrowia lipolytica UCDFST 51-30, but decreased from 5 to 65% in other yeasts. An equimolar concentration of the chloride salt resulted in much milder effects, from 25% decrease to 66% increase in lipid output. The highest lipid outputs in this media were 8.3 and 7.9 g/L produced by Vanrija humicola UCDFST 10-1004 and UCDFST 12-717, respectively. These results demonstrated substantial lipid production in the presence of [C2C1Im]Cl at concentrations found in lignocellulosic hydrolysates, and thus, these two strains are ideal candidates for further investigation. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 25% |
| United States | 1 | 25% |
| France | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 22% |
| Researcher | 6 | 16% |
| Student > Doctoral Student | 3 | 8% |
| Professor | 3 | 8% |
| Student > Master | 3 | 8% |
| Other | 4 | 11% |
| Unknown | 10 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 24% |
| Biochemistry, Genetics and Molecular Biology | 6 | 16% |
| Engineering | 3 | 8% |
| Medicine and Dentistry | 2 | 5% |
| Environmental Science | 1 | 3% |
| Other | 5 | 14% |
| Unknown | 11 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 December 2017.
All research outputs
#14,350,314
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#5,305
of 8,034 outputs
Outputs of similar age
#169,857
of 331,121 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#56
of 129 outputs
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