| Title |
Development of Adenosine Deaminase-Specific IgY Antibodies: Diagnostic and Inhibitory Application
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, October 2017
|
| DOI | 10.1007/s12010-017-2626-x |
| Pubmed ID | |
| Authors |
Agnieszka Łupicka-Słowik, Mateusz Psurski, Renata Grzywa, Kamila Bobrek, Patrycja Smok, Maciej Walczak, Andrzej Gaweł, Tadeusz Stefaniak, Józef Oleksyszyn, Marcin Sieńczyk |
| Abstract |
Adenosine deaminase (ADA) is currently used as a diagnostic marker for tuberculous pleuritis. Although ADA has been suggested as a potential marker for several types of cancer, the importance of each of ADA isoforms as well as their levels and enzymatic activities in tumors need to be further investigated. Herein we developed avian immunoglobulin Y highly specific to human ADA via hens immunization with calf adenosine deaminase. The obtained antibodies were used for the development of a sensitive double-egg yolk immunoglobulin (IgY) sandwich ELISA assay with an ADA detection limit of 0.5 ng/ml and a linearity range of up to 10 ng/ml. Specific, affinity-purified IgYs were able to recognize human recombinant ADA and ADA present in human cancer cell lines. In addition, antigen-specific IgY antibodies were able to inhibit catalytic activity of calf ADA with an IC50 value of 47.48 nM. We showed that generated IgY antibodies may be useful for ADA detection, thus acting as a diagnostic agent in immunoenzymatic assays. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 36 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 17% |
| Student > Ph. D. Student | 5 | 14% |
| Student > Master | 5 | 14% |
| Researcher | 3 | 8% |
| Professor | 2 | 6% |
| Other | 7 | 19% |
| Unknown | 8 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 33% |
| Immunology and Microbiology | 4 | 11% |
| Agricultural and Biological Sciences | 3 | 8% |
| Chemistry | 2 | 6% |
| Veterinary Science and Veterinary Medicine | 2 | 6% |
| Other | 3 | 8% |
| Unknown | 10 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 October 2017.
All research outputs
#18,574,814
of 23,006,268 outputs
Outputs from Applied Biochemistry and Biotechnology
#1,794
of 2,527 outputs
Outputs of similar age
#250,288
of 326,544 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#16
of 40 outputs
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