Title |
Recapitulating cortical development with organoid culture in vitro and modeling abnormal spindle-like (ASPM related primary) microcephaly disease
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Published in |
Protein & Cell, October 2017
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DOI | 10.1007/s13238-017-0479-2 |
Pubmed ID | |
Authors |
Rui Li, Le Sun, Ai Fang, Peng Li, Qian Wu, Xiaoqun Wang |
Abstract |
The development of a cerebral organoid culture in vitro offers an opportunity to generate human brain-like organs to investigate mechanisms of human disease that are specific to the neurogenesis of radial glial (RG) and outer radial glial (oRG) cells in the ventricular zone (VZ) and subventricular zone (SVZ) of the developing neocortex. Modeling neuronal progenitors and the organization that produces mature subcortical neuron subtypes during early stages of development is essential for studying human brain developmental diseases. Several previous efforts have shown to grow neural organoid in culture dishes successfully, however we demonstrate a new paradigm that recapitulates neocortical development process with VZ, OSVZ formation and the lamination organization of cortical layer structure. In addition, using patient-specific induced pluripotent stem cells (iPSCs) with dysfunction of the Aspm gene from a primary microcephaly patient, we demonstrate neurogenesis defects result in defective neuronal activity in patient organoids, suggesting a new strategy to study human developmental diseases in central nerve system. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 196 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 40 | 20% |
Researcher | 29 | 15% |
Student > Bachelor | 26 | 13% |
Student > Master | 22 | 11% |
Student > Doctoral Student | 10 | 5% |
Other | 17 | 9% |
Unknown | 52 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 42 | 21% |
Biochemistry, Genetics and Molecular Biology | 35 | 18% |
Agricultural and Biological Sciences | 31 | 16% |
Medicine and Dentistry | 13 | 7% |
Engineering | 6 | 3% |
Other | 12 | 6% |
Unknown | 57 | 29% |