| Title |
Nuclear Wiskott–Aldrich syndrome protein co-regulates T cell factor 1-mediated transcription in T cells
|
|---|---|
| Published in |
Genome Medicine, October 2017
|
| DOI | 10.1186/s13073-017-0481-6 |
| Pubmed ID | |
| Authors |
Nikolai V. Kuznetsov, Bader Almuzzaini, Joanna S. Kritikou, Marisa A. P. Baptista, Mariana M. S. Oliveira, Marton Keszei, Scott B. Snapper, Piergiorgio Percipalle, Lisa S. Westerberg |
| Abstract |
The Wiskott-Aldrich syndrome protein (WASp) family of actin-nucleating factors are present in the cytoplasm and in the nucleus. The role of nuclear WASp for T cell development remains incompletely defined. We performed WASp chromatin immunoprecipitation and deep sequencing (ChIP-seq) in thymocytes and spleen CD4(+) T cells. WASp was enriched at genic and intergenic regions and associated with the transcription start sites of protein-coding genes. Thymocytes and spleen CD4(+) T cells showed 15 common WASp-interacting genes, including the gene encoding T cell factor (TCF)12. WASp KO thymocytes had reduced nuclear TCF12 whereas thymocytes expressing constitutively active WASp(L272P) and WASp(I296T) had increased nuclear TCF12, suggesting that regulated WASp activity controlled nuclear TCF12. We identify a putative DNA element enriched in WASp ChIP-seq samples identical to a TCF1-binding site and we show that WASp directly interacted with TCF1 in the nucleus. These data place nuclear WASp in proximity with TCF1 and TCF12, essential factors for T cell development. |
X Demographics
The data shown below were collected from the profiles of 12 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 17% |
| Mexico | 1 | 8% |
| Brunei Darussalam | 1 | 8% |
| Saudi Arabia | 1 | 8% |
| Unknown | 7 | 58% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 50% |
| Scientists | 3 | 25% |
| Science communicators (journalists, bloggers, editors) | 2 | 17% |
| Practitioners (doctors, other healthcare professionals) | 1 | 8% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 25% |
| Student > Bachelor | 4 | 13% |
| Student > Master | 4 | 13% |
| Researcher | 4 | 13% |
| Professor | 2 | 6% |
| Other | 4 | 13% |
| Unknown | 6 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 31% |
| Agricultural and Biological Sciences | 5 | 16% |
| Immunology and Microbiology | 4 | 13% |
| Medicine and Dentistry | 3 | 9% |
| Engineering | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 6 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 March 2018.
All research outputs
#5,151,804
of 25,225,928 outputs
Outputs from Genome Medicine
#955
of 1,560 outputs
Outputs of similar age
#84,496
of 335,118 outputs
Outputs of similar age from Genome Medicine
#20
of 27 outputs
Altmetric has tracked 25,225,928 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,560 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.1. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 335,118 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.