| Title |
One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells
|
|---|---|
| Published in |
Stem Cell Reviews and Reports, September 2015
|
| DOI | 10.1007/s12015-015-9621-9 |
| Pubmed ID | |
| Authors |
Annika Asplund, Arvind Pradip, Mariska van Giezen, Anders Aspegren, Helena Choukair, Marie Rehnström, Susanna Jacobsson, Nidal Ghosheh, Dorra El Hajjam, Sandra Holmgren, Susanna Larsson, Jörg Benecke, Mariela Butron, Annelie Wigander, Karin Noaksson, Peter Sartipy, Petter Björquist, Josefina Edsbagge, Barbara Küppers-Munther |
| Abstract |
Human hepatocytes display substantial functional inter-individual variation regarding drug metabolizing functions. In order to investigate if this diversity is mirrored in hepatocytes derived from different human pluripotent stem cell (hPSC) lines, we evaluated 25 hPSC lines originating from 24 different donors for hepatic differentiation and functionality. Homogenous hepatocyte cultures could be derived from all hPSC lines using one standardized differentiation procedure. To the best of our knowledge this is the first report of a standardized hepatic differentiation procedure that is generally applicable across a large panel of hPSC lines without any adaptations to individual lines. Importantly, with regard to functional aspects, such as Cytochrome P450 activities, we observed that hepatocytes derived from different hPSC lines displayed inter-individual variation characteristic for primary hepatocytes obtained from different donors, while these activities were highly reproducible between repeated experiments using the same line. Taken together, these data demonstrate the emerging possibility to compile panels of hPSC-derived hepatocytes of particular phenotypes/genotypes relevant for drug metabolism and toxicity studies. Moreover, these findings are of significance for applications within the regenerative medicine field, since our stringent differentiation procedure allows the derivation of homogenous hepatocyte cultures from multiple donors which is a prerequisite for the realization of future personalized stem cell based therapies. |
Mendeley readers
The data shown below were compiled from readership statistics for 96 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 1% |
| Unknown | 95 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 19% |
| Student > Ph. D. Student | 17 | 18% |
| Student > Master | 12 | 13% |
| Student > Bachelor | 7 | 7% |
| Other | 7 | 7% |
| Other | 16 | 17% |
| Unknown | 19 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 25 | 26% |
| Agricultural and Biological Sciences | 23 | 24% |
| Pharmacology, Toxicology and Pharmaceutical Science | 10 | 10% |
| Medicine and Dentistry | 9 | 9% |
| Immunology and Microbiology | 2 | 2% |
| Other | 9 | 9% |
| Unknown | 18 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 July 2023.
All research outputs
#3,710,762
of 25,374,917 outputs
Outputs from Stem Cell Reviews and Reports
#111
of 1,036 outputs
Outputs of similar age
#47,921
of 284,613 outputs
Outputs of similar age from Stem Cell Reviews and Reports
#4
of 15 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,036 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done well, scoring higher than 88% of its peers.
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We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.