| Title |
Identification and quantification of cannabinoids in Cannabis sativa L. plants by high performance liquid chromatography-mass spectrometry
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, October 2014
|
| DOI | 10.1007/s00216-014-8177-x |
| Pubmed ID | |
| Authors |
Oier Aizpurua-Olaizola, Jone Omar, Patricia Navarro, Maitane Olivares, Nestor Etxebarria, Aresatz Usobiaga |
| Abstract |
High performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) has been successfully applied to cannabis plant extracts in order to identify cannabinoid compounds after their quantitative isolation by means of supercritical fluid extraction (SFE). MS conditions were optimized by means of a central composite design (CCD) approach, and the analysis method was fully validated. Six major cannabinoids [tetrahydrocannabinolic acid (THCA), tetrahydrocannabinol (THC), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabigerol (CBG), and cannabinol (CBN)] were quantified (RSD < 10%), and seven more cannabinoids were identified and verified by means of a liquid chromatograph coupled to a quadrupole-time-of-flight (Q-ToF) detector. Finally, based on the distribution of the analyzed cannabinoids in 30 Cannabis sativa L. plant varieties and the principal component analysis (PCA) of the resulting data, a clear difference was observed between outdoor and indoor grown plants, which was attributed to a higher concentration of THC, CBN, and CBD in outdoor grown plants. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 29% |
| United Kingdom | 1 | 14% |
| Argentina | 1 | 14% |
| Unknown | 3 | 43% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 71% |
| Scientists | 1 | 14% |
| Practitioners (doctors, other healthcare professionals) | 1 | 14% |
Mendeley readers
The data shown below were compiled from readership statistics for 415 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | 2% |
| United Kingdom | 1 | <1% |
| South Africa | 1 | <1% |
| Austria | 1 | <1% |
| Unknown | 405 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 64 | 15% |
| Student > Master | 63 | 15% |
| Student > Ph. D. Student | 54 | 13% |
| Student > Bachelor | 53 | 13% |
| Other | 16 | 4% |
| Other | 37 | 9% |
| Unknown | 128 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 85 | 20% |
| Agricultural and Biological Sciences | 54 | 13% |
| Biochemistry, Genetics and Molecular Biology | 40 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 28 | 7% |
| Medicine and Dentistry | 14 | 3% |
| Other | 50 | 12% |
| Unknown | 144 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2024.
All research outputs
#2,149,523
of 25,394,764 outputs
Outputs from Analytical & Bioanalytical Chemistry
#132
of 9,624 outputs
Outputs of similar age
#24,323
of 273,407 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#3
of 107 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,624 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 273,407 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 107 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.