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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Nanopores Discriminate among Five C5-Cytosine Variants in DNA
|
|---|---|
| Published in |
Journal of the American Chemical Society, November 2014
|
| DOI | 10.1021/ja508527b |
| Pubmed ID | |
| Authors |
Zachary L. Wescoe, Jacob Schreiber, Mark Akeson |
| Abstract |
Individual DNA molecules can be read at single nucleotide precision using nanopores coupled to processive enzymes. Discrimination among the four canonical bases has been achieved, as has discrimination among cytosine, 5-methylcytosine (mC), and 5-hydroxymethylcytosine (hmC). Two additional modified cytosine bases, 5-carboxylcytosine (caC) and 5-formylcytosine (fC), are produced during enzymatic conversion of hmC to cytosine in mammalian cells. Thus an accurate picture of cytosine epigenetic status in target cells must also include these C5-cytosine variants. In the present study, we used a patch clamp amplifier to acquire ionic current traces caused by phi29 DNA polymerase-controlled translocation of DNA templates through the M2MspA pore. Decision boundaries based on three consecutive ionic current states were implemented to call mC, hmC, caC, fC or cytosine at CG dinucleotides in ~ 4,400 individual DNA molecules. We found that the percentage of correct base calls for single pass reads ranged from 91.6% to 98.3%. This accuracy depended upon the identity of nearest neighbor bases surrounding the CG dinucleotide. |
X Demographics
The data shown below were collected from the profiles of 20 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 25% |
| United Kingdom | 3 | 15% |
| France | 2 | 10% |
| Australia | 1 | 5% |
| Switzerland | 1 | 5% |
| Unknown | 8 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 50% |
| Scientists | 8 | 40% |
| Practitioners (doctors, other healthcare professionals) | 1 | 5% |
| Science communicators (journalists, bloggers, editors) | 1 | 5% |
Mendeley readers
The data shown below were compiled from readership statistics for 206 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | <1% |
| Korea, Republic of | 1 | <1% |
| India | 1 | <1% |
| Belgium | 1 | <1% |
| United Kingdom | 1 | <1% |
| Japan | 1 | <1% |
| Russia | 1 | <1% |
| Unknown | 198 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 51 | 25% |
| Researcher | 30 | 15% |
| Student > Master | 28 | 14% |
| Student > Bachelor | 24 | 12% |
| Professor > Associate Professor | 11 | 5% |
| Other | 28 | 14% |
| Unknown | 34 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 57 | 28% |
| Agricultural and Biological Sciences | 35 | 17% |
| Chemistry | 26 | 13% |
| Engineering | 15 | 7% |
| Physics and Astronomy | 12 | 6% |
| Other | 25 | 12% |
| Unknown | 36 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 21. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2024.
All research outputs
#1,751,412
of 25,312,451 outputs
Outputs from Journal of the American Chemical Society
#3,148
of 66,338 outputs
Outputs of similar age
#19,468
of 265,262 outputs
Outputs of similar age from Journal of the American Chemical Society
#34
of 528 outputs
Altmetric has tracked 25,312,451 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 66,338 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.2. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,262 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 528 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.