Title |
The transcriptome of human pluripotent stem cells
|
---|---|
Published in |
Current Opinion in Genetics & Development, October 2014
|
DOI | 10.1016/j.gde.2014.09.012 |
Pubmed ID | |
Authors |
Kin Fai Au, Vittorio Sebastiano |
Abstract |
Human Embryonic Stem Cells (hESCs) are in vitro derivatives of the inner cell mass of the blastocyst and are characterized by an undifferentiated and pluripotent state that can be perpetuated in time, indefinitely. hESCs provide a unique opportunity to both dissect the molecular mechanisms that are predisposed to the maintenance of pluripotency and model the ability to initiate differentiation and cell commitment within the developing embryo. To fully understand these mechanisms, it is necessary to accurately identify the specific transcriptome of hESCs. Many distinct gene annotation methods, such as cDNA and EST sequencing and RNA-Seq, have been used to identify the transcriptome of hESCs. Lately, we developed a new tool (IDP) to integrate the hybrid sequencing data to characterize a more reliable and comprehensive hESC transcriptome with discoveries of many novel transcripts. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 20% |
United Kingdom | 1 | 20% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
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Scientists | 3 | 60% |
Members of the public | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 3% |
United States | 1 | 1% |
India | 1 | 1% |
Germany | 1 | 1% |
Unknown | 65 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 22 | 31% |
Researcher | 16 | 23% |
Student > Master | 11 | 16% |
Student > Bachelor | 5 | 7% |
Other | 5 | 7% |
Other | 11 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 35 | 50% |
Biochemistry, Genetics and Molecular Biology | 12 | 17% |
Medicine and Dentistry | 7 | 10% |
Social Sciences | 2 | 3% |
Neuroscience | 2 | 3% |
Other | 10 | 14% |
Unknown | 2 | 3% |