Title |
Influence of genetic background, salinity, and inoculum size on growth of the ichthyotoxic golden alga (Prymnesium parvum)
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Published in |
Harmful Algae, June 2017
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DOI | 10.1016/j.hal.2017.05.010 |
Pubmed ID | |
Authors |
Rakib H. Rashel, Reynaldo Patiño |
Abstract |
Salinity (5-30) effects on golden alga growth were determined at a standard laboratory temperature (22°C) and one associated with natural blooms (13°C). Inoculum-size effects were determined over a wide size range (100-100,000cellsml(-1)). A strain widely distributed in the USA, UTEX-2797 was the primary study subject but another of limited distribution, UTEX-995 was used to evaluate growth responses in relation to genetic background. Variables examined were exponential growth rate (r), maximum cell density (max-D) and, when inoculum size was held constant (100cellsml(-1)), density at onset of exponential growth (early-D). In UTEX-2797, max-D increased as salinity increased from 5 to ∼10-15 and declined thereafter regardless of temperature but r remained generally stable and only declined at salinity of 25-30. In addition, max-D correlated positively with r and early-D, the latter also being numerically highest at salinity of 15. In UTEX-995, max-D and r responded similarly to changes in salinity - they remained stable at salinity of 5-10 and 5-15, respectively, and declined at higher salinity. Also, max-D correlated with r but not early-D. Inoculum size positively and negatively influenced max-D and r, respectively, in both strains and these effects were significant even when the absolute size difference was small (100 versus 1000 cells ml(-1)). When cultured under similar conditions, UTEX-2797 grew faster and to much higher density than UTEX-995. In conclusion, (1) UTEX-2797's superior growth performance may explain its relatively wide distribution in the USA, (2) the biphasic growth response of UTEX-2797 to salinity variation, with peak abundance at salinity of 10-15, generally mirrors golden alga abundance-salinity associations in US inland waters, and (3) early cell density - whether artificially manipulated or naturally attained - can influence UTEX-2797 bloom potential. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 23% |
Researcher | 5 | 17% |
Student > Master | 4 | 13% |
Professor | 2 | 7% |
Lecturer | 2 | 7% |
Other | 2 | 7% |
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Biochemistry, Genetics and Molecular Biology | 4 | 13% |
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Engineering | 2 | 7% |
Other | 3 | 10% |
Unknown | 10 | 33% |