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Identification of novel cis-regulatory elements of Eya1 in Xenopus laevis using BAC recombineering

Overview of attention for article published in Scientific Reports, November 2017
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Title
Identification of novel cis-regulatory elements of Eya1 in Xenopus laevis using BAC recombineering
Published in
Scientific Reports, November 2017
DOI 10.1038/s41598-017-15153-7
Pubmed ID
Authors

Santosh Kumar Maharana, Nicolas Pollet, Gerhard Schlosser

Abstract

The multifunctional Eya1 protein plays important roles during the development of cranial sensory organs and ganglia, kidneys, hypaxial muscles and several other organs in vertebrates. Eya1 is encoded by a complex locus with candidate cis-regulatory elements distributed over a 329 kbp wide genomic region in Xenopus. Consequently, very little is currently known about how expression of Eya1 is controlled by upstream regulators. Here we use a library of Xenopus tropicalis genomic sequences in bacterial artificial chromosomes (BAC) to analyze the genomic region surrounding the Eya1 locus for enhancer activity. We used BAC recombineering to first create GFP reporter constructs, which were analysed for enhancer activity by injection into Xenopus laevis embryos. We then used a second round of BAC recombineering to create deletion constructs of these BAC reporters to localize enhancer activity more precisely. This double recombineering approach allowed us to probe a large genomic region for enhancer activity without assumptions on sequence conservation. Using this approach we were able to identify two novel cis-regulatory regions, which direct Eya1 expression to the somites, pharyngeal pouches, the preplacodal ectoderm (the common precursor region of many cranial sensory organs and ganglia), and other ectodermal domains.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 27%
Student > Master 2 18%
Student > Bachelor 1 9%
Student > Doctoral Student 1 9%
Student > Ph. D. Student 1 9%
Other 0 0%
Unknown 3 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 45%
Biochemistry, Genetics and Molecular Biology 2 18%
Unknown 4 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 November 2017.
All research outputs
#13,572,617
of 23,007,053 outputs
Outputs from Scientific Reports
#61,560
of 124,255 outputs
Outputs of similar age
#166,425
of 329,030 outputs
Outputs of similar age from Scientific Reports
#2,042
of 4,447 outputs
Altmetric has tracked 23,007,053 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 124,255 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.2. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 329,030 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4,447 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.