| Title |
Identification of nestin-positive putative mammary stem cells in human breastmilk
|
|---|---|
| Published in |
Cell and Tissue Research, April 2007
|
| DOI | 10.1007/s00441-007-0390-x |
| Pubmed ID | |
| Authors |
Mark D. Cregan, Yiping Fan, Amber Appelbee, Mark L. Brown, Borut Klopcic, John Koppen, Leon R. Mitoulas, Kristin M. E. Piper, Mahesh A Choolani, Yap-Seng Chong, Peter E. Hartmann |
| Abstract |
Stem cells in mammary tissue have been well characterised by using the mammary stem cell marker, cytokeratin (CK) 5 and the mature epithelial markers CK14, CK18 and CK19. As these markers have never been reported in cells from breastmilk, the aim of this study has been to determine whether mammary stem cells are present in expressed human breastmilk. Cultured cells from human breastmilk were studied by using immunofluorescent labelling and reverse transcription/polymerase chain reaction (RT-PCR). We found a heterogeneous population of cells with differential expression of CK5, CK14, CK18 and CK19. Further, by using the multipotent stem cell marker, nestin, we identified cells in culture that were positive only for nestin or double-positive for CK5/nestin, whereas no co-staining was observed for CK14, CK18 and CK19 with nestin. When cells isolated from breastmilk were analysed by using RT-PCR prior to culture, only nestin and CK18 were detected, thereby indicating that breastmilk contained differentiated epithelial and putative stem cells. Furthermore, fluorescence-activated cell-sorting analysis demonstrated, in breastmilk, a small side-population of cells that excluded Hoechst 33342 (a key property of multipotent stem cells). When stained for nestin, the cells in the side-population were positive, whereas those not in the side-population were negative. The presence of nestin-positive putative mammary stem cells suggests that human breastmilk is a readily available and non-invasive source of putative mammary stem cells that may be useful for research into both mammary gland biology and more general stem cell biology. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 111 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | <1% |
| India | 1 | <1% |
| Poland | 1 | <1% |
| Germany | 1 | <1% |
| Unknown | 107 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 15% |
| Student > Ph. D. Student | 16 | 14% |
| Student > Bachelor | 11 | 10% |
| Student > Master | 9 | 8% |
| Other | 7 | 6% |
| Other | 29 | 26% |
| Unknown | 22 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 22% |
| Medicine and Dentistry | 20 | 18% |
| Biochemistry, Genetics and Molecular Biology | 19 | 17% |
| Immunology and Microbiology | 4 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 4% |
| Other | 15 | 14% |
| Unknown | 25 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 22. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2016.
All research outputs
#1,549,619
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#17
of 2,279 outputs
Outputs of similar age
#3,018
of 75,507 outputs
Outputs of similar age from Cell and Tissue Research
#1
of 14 outputs
Altmetric has tracked 23,839,820 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,279 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 99% of its peers.
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We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.