| Title |
In Vitro Antimicrobial Activity and Downregulation of Virulence Gene Expression on Helicobacter pylori by Reuterin
|
|---|---|
| Published in |
Probiotics and Antimicrobial Proteins, November 2017
|
| DOI | 10.1007/s12602-017-9342-2 |
| Pubmed ID | |
| Authors |
Víctor Hugo Urrutia-Baca, Erandi Escamilla-García, Myriam Angélica de la Garza-Ramos, Patricia Tamez-Guerra, Ricardo Gomez-Flores, Cynthia Sofía Urbina-Ríos |
| Abstract |
Helicobacter pylori is an infectious agent commonly associated with gastrointestinal diseases. The use of probiotics to treat this infection has been documented, however, their potential antimicrobial metabolites have not yet been investigated. In the present study, the effect of reuterin produced by Lactobacillus reuteri on H. pylori growth and virulence gene expression was evaluated. It was observed that reuterin caused significant (P < 0.05) H. pylori growth inhibition at concentrations from 0.08 to 20.48 mM, with minimal inhibitory concentrations (MICs) of 20.48 mM for H. pylori ATCC700824 and 10.24 mM for H. pylori ATCC43504. In a reuterin bacterial killing assay, it was observed that half of the MIC value for H. pylori (ATCC700824) significantly (P < 0.01) reduced colony numbers from 5.65 ± 0.35 to 3.78 ± 0.35 Log10 CFU/mL after 12 h of treatment and then increased them to 5.25 ± 0.23 Log10 CFU/mL at 24 h; at its MIC value (20.48 mM), reuterin abrogated (P < 0.01) H. pylori (ATCC700824) growth after 20 h of culture. In addition, reuterin significantly (P < 0.01) reduced H. pylori (ATCC 43504) colony numbers from 5.65 ± 0.35 to 4.1 ± 0.12 Log10 CFU/mL from 12 to 24 h of treatment and abrogated its growth at its MIC value (10.24 mM), after 20 h of treatment. Reuterin did not alter normal human gastric Hs738.St/Int cell viability at the concentrations tested for H. pylori strains. Furthermore, 10 μM reuterin was shown to significantly (P < 0.01) reduce mRNA relative expression levels of H. pylori virulence genes vacA and flaA at 3 h post-treatment, whose effect was higher at 6 h post-treatment, as measured by RT-qPCR. The observed direct antimicrobial effect and the downregulation of expression of virulence genes on H. pylori by reuterin may contribute to the understanding of the mechanisms of action of probiotics against H. pylori. |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 71 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 14% |
| Student > Ph. D. Student | 8 | 11% |
| Researcher | 7 | 10% |
| Student > Bachelor | 7 | 10% |
| Student > Doctoral Student | 3 | 4% |
| Other | 9 | 13% |
| Unknown | 27 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 17% |
| Immunology and Microbiology | 9 | 13% |
| Medicine and Dentistry | 8 | 11% |
| Biochemistry, Genetics and Molecular Biology | 6 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 1% |
| Other | 4 | 6% |
| Unknown | 31 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 December 2017.
All research outputs
#20,451,228
of 23,007,053 outputs
Outputs from Probiotics and Antimicrobial Proteins
#399
of 545 outputs
Outputs of similar age
#287,525
of 330,012 outputs
Outputs of similar age from Probiotics and Antimicrobial Proteins
#18
of 25 outputs
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