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High Efficiency Ex Vivo Cloning of Antigen-Specific Human Effector T Cells

Overview of attention for article published in PLOS ONE, November 2014
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Title
High Efficiency Ex Vivo Cloning of Antigen-Specific Human Effector T Cells
Published in
PLOS ONE, November 2014
DOI 10.1371/journal.pone.0110741
Pubmed ID
Authors

Michelle A. Neller, Michael H.-L. Lai, Catherine M. Lanagan, Linda E. O′Connor, Antonia L. Pritchard, Nathan R. Martinez, Christopher W. Schmidt

Abstract

While cloned T cells are valuable tools for the exploration of immune responses against viruses and tumours, current cloning methods do not allow inferences to be made about the function and phenotype of a clone's in vivo precursor, nor can precise cloning efficiencies be calculated. Additionally, there is currently no general method for cloning antigen-specific effector T cells directly from peripheral blood mononuclear cells, without the need for prior expansion in vitro. Here we describe an efficient method for cloning effector T cells ex vivo. Functional T cells are detected using optimised interferon gamma capture following stimulation with viral or tumour cell-derived antigen. In combination with multiple phenotypic markers, single effector T cells are sorted using a flow cytometer directly into multi-well plates, and cloned using standard, non antigen-specific expansion methods. We provide examples of this novel technology to generate antigen-reactive clones from healthy donors using Epstein-Barr virus and cytomegalovirus as representative viral antigen sources, and from two melanoma patients using autologous melanoma cells. Cloning efficiency, clonality, and retention/loss of function are described. Ex vivo effector cell cloning provides a rapid and effective method of deriving antigen-specific T cells clones with traceable in vivo precursor function and phenotype.

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The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Argentina 1 2%
Unknown 65 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 19 29%
Student > Ph. D. Student 12 18%
Student > Bachelor 6 9%
Other 6 9%
Student > Master 5 8%
Other 8 12%
Unknown 10 15%
Readers by discipline Count As %
Immunology and Microbiology 19 29%
Biochemistry, Genetics and Molecular Biology 12 18%
Agricultural and Biological Sciences 12 18%
Medicine and Dentistry 9 14%
Business, Management and Accounting 1 2%
Other 4 6%
Unknown 9 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 November 2014.
All research outputs
#13,866,924
of 23,508,125 outputs
Outputs from PLOS ONE
#113,646
of 201,322 outputs
Outputs of similar age
#127,655
of 263,792 outputs
Outputs of similar age from PLOS ONE
#2,386
of 5,091 outputs
Altmetric has tracked 23,508,125 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 201,322 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.3. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,792 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 5,091 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.