| Title |
The Class-A GPCR Dopamine D2 Receptor Forms Transient Dimers Stabilized by Agonists: Detection by Single-Molecule Tracking
|
|---|---|
| Published in |
Cell Biochemistry and Biophysics, November 2017
|
| DOI | 10.1007/s12013-017-0829-y |
| Pubmed ID | |
| Authors |
Rinshi S. Kasai, Shuichi V. Ito, Ryo M. Awane, Takahiro K. Fujiwara, Akihiro Kusumi |
| Abstract |
Whether class-A G-protein coupled receptors (GPCRs) exist and work as monomers or dimers has drawn extensive attention. A class-A GPCR dopamine D2 receptor (D2R) is involved in many physiological and pathological processes and diseases, indicating its critical role in proper functioning of neuronal circuits. In particular, D2R homodimers might play key roles in schizophrenia development and amphetamine-induced psychosis. Here, using single-molecule imaging, we directly tracked single D2R molecules in the plasma membrane at a physiological temperature of 37 °C, and unequivocally determined that D2R forms transient dimers with a lifetime of 68 ms in its resting state. Agonist addition prolonged the dimer lifetime by a factor of ~1.5, suggesting the possibility that transient dimers might be involved in signaling. |
X Demographics
The data shown below were collected from the profiles of 17 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 18% |
| United States | 2 | 12% |
| Japan | 2 | 12% |
| France | 1 | 6% |
| Australia | 1 | 6% |
| India | 1 | 6% |
| Turkey | 1 | 6% |
| Switzerland | 1 | 6% |
| Denmark | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 4 | 24% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 59% |
| Scientists | 7 | 41% |
Mendeley readers
The data shown below were compiled from readership statistics for 96 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 96 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 23% |
| Researcher | 18 | 19% |
| Student > Bachelor | 9 | 9% |
| Student > Master | 9 | 9% |
| Other | 4 | 4% |
| Other | 7 | 7% |
| Unknown | 27 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 26 | 27% |
| Agricultural and Biological Sciences | 10 | 10% |
| Chemistry | 7 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 5% |
| Neuroscience | 3 | 3% |
| Other | 11 | 11% |
| Unknown | 34 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 November 2017.
All research outputs
#3,928,775
of 24,577,646 outputs
Outputs from Cell Biochemistry and Biophysics
#40
of 949 outputs
Outputs of similar age
#67,672
of 336,981 outputs
Outputs of similar age from Cell Biochemistry and Biophysics
#1
of 12 outputs
Altmetric has tracked 24,577,646 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 949 research outputs from this source. They receive a mean Attention Score of 2.1. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 336,981 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.