| Title |
Effect of methylene blue-mediated antimicrobial photodynamic therapy on dentin caries microcosms
|
|---|---|
| Published in |
Lasers in Medical Science, November 2017
|
| DOI | 10.1007/s10103-017-2379-3 |
| Pubmed ID | |
| Authors |
Daniela Alejandra Cusicanqui Méndez, Eliezer Gutierrez, Evandro José Dionísio, Thaís Marchini Oliveira, Marília Afonso Rabelo Buzalaf, Daniela Rios, Maria Aparecida Andrade Moreira Machado, Thiago Cruvinel |
| Abstract |
Antimicrobial photodynamic therapy (aPDT) has been proposed as an adjuvant treatment of dental caries, although there are no well-defined protocols to its clinical application. This study aimed to evaluate the influence of aPDT on the viability of microorganisms, vitality of biofilms, and lactic acid production of dentin caries microcosms. Biofilms were grown on bovine dentin discs in anaerobic conditions at 37 °C for 5 days, inoculating infected carious dentin in modified McBain medium plus 1% sucrose. The biofilms were treated by the combination of deionized water or 100 mg L(-1) methylene blue (MB) with 0, 37.5, or 75 J cm(-2) LED at 630 nm. The counts of total microorganisms, total streptococci, mutans streptococci, and total lactobacilli were determined by colony-forming units (CFU). The vitality of microbial cells in intact biofilms was measured by confocal laser scanning microscope (CLSM). The lactic acid production was analyzed by enzymatic spectrophotometry at 340 nm. Statistical analysis was conducted by Kruskal-Wallis and post hoc Dunn's tests (P < 0.05). MB and 37.5 J cm(-2) LED alone did not interfere in the viability of microorganisms, unlike 75 J cm(-2) LED alone that decreased the total microorganism and lactobacillus counts. The combination of MB and 75 J cm(-2) LED reduced the viability of all microorganisms and the vitality of intact biofilms. The production of lactic acid was statistically lower in all treatment groups in comparison with that of the control (no treatment), except for MB alone. Therefore, the MB-mediated aPDT was effective in controlling the viability, vitality and the acidogenicity of dentin caries microcosms. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 76 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 14 | 18% |
| Student > Bachelor | 11 | 14% |
| Student > Ph. D. Student | 10 | 13% |
| Other | 7 | 9% |
| Professor > Associate Professor | 4 | 5% |
| Other | 9 | 12% |
| Unknown | 21 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 31 | 41% |
| Biochemistry, Genetics and Molecular Biology | 6 | 8% |
| Agricultural and Biological Sciences | 3 | 4% |
| Immunology and Microbiology | 3 | 4% |
| Chemistry | 2 | 3% |
| Other | 6 | 8% |
| Unknown | 25 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2017.
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#20,451,991
of 23,007,887 outputs
Outputs from Lasers in Medical Science
#1,086
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#288,901
of 331,435 outputs
Outputs of similar age from Lasers in Medical Science
#31
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