| Title |
The apicomplexan parasite Eimeria arloingi induces caprine neutrophil extracellular traps
|
|---|---|
| Published in |
Parasitology Research, May 2014
|
| DOI | 10.1007/s00436-014-3939-0 |
| Pubmed ID | |
| Authors |
Liliana M. R. Silva, Tamara Muñoz Caro, Rüdiger Gerstberger, Maria J. M. Vila-Viçosa, Helder C. E. Cortes, Carlos Hermosilla, Anja Taubert |
| Abstract |
As a novel effector mechanism polymorphonuclear neutrophils (PMN) release neutrophil extracellular traps (NETs), which represent protein-labeled DNA matrices capable of extracellular trapping and killing of invasive pathogens. Here, we demonstrate for the first time NET formation performed by caprine PMN exposed to different stages (sporozoites and oocysts) of the goat apicomplexan protozoan parasite Eimeria arloingi. Scanning electron microscopy as well as fluorescence microscopy of sporozoites- and oocysts-PMN co-cultures revealed a fine network of DNA fibrils partially covering the parasites. Immunofluorescence analyses confirmed the co-localization of histones (H3), neutrophil elastase (NE), and myeloperoxidase (MPO) in extracellular traps released from caprine PMN. In addition, the enzymatic activity of NE was found significantly enhanced in sporozoite-exposed caprine PMN. The treatment of caprine NET structures with deoxyribonuclease (DNase) and the NADPH oxidase inhibitor diphenylene iodondium (DPI) significantly reduced NETosis confirming the classical characteristics of NETs. Caprine NETs efficiently trapped vital sporozoites of E. arloingi since 72% of these stages were immobilized-but not killed-in NET structures. As a consequence, early infection rates were significantly reduced when PMN-pre-exposed sporozoites were allowed to infect adequate host cells. These findings suggest that NETs may play an important role in the early innate host response to E. arloingi infection in goats. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 47 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 23% |
| Researcher | 7 | 15% |
| Student > Master | 7 | 15% |
| Professor > Associate Professor | 4 | 9% |
| Student > Doctoral Student | 3 | 6% |
| Other | 6 | 13% |
| Unknown | 9 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 30% |
| Biochemistry, Genetics and Molecular Biology | 7 | 15% |
| Immunology and Microbiology | 6 | 13% |
| Veterinary Science and Veterinary Medicine | 5 | 11% |
| Medicine and Dentistry | 2 | 4% |
| Other | 0 | 0% |
| Unknown | 13 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 November 2014.
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#20,242,779
of 22,770,070 outputs
Outputs from Parasitology Research
#2,877
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#191,923
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Outputs of similar age from Parasitology Research
#38
of 63 outputs
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