Catalase and ascorbate peroxidase—representative H2O2-detoxifying heme enzymes in plants

Naser A. Anjum, Pallavi Sharma, Sarvajeet S. Gill, Mirza Hasanuzzaman, Ekhlaque A. Khan, Kiran Kachhap, Amal A. Mohamed, Palaniswamy Thangavel, Gurumayum Devmanjuri Devi, Palanisamy Vasudhevan, Adriano Sofo, Nafees A. Khan, Amarendra Narayan Misra, Alexander S. Lukatkin, Harminder Pal Singh, Eduarda Pereira, Narendra Tuteja

Plants have to counteract unavoidable stress-caused anomalies such as oxidative stress to sustain their lives and serve heterotrophic organisms including humans. Among major enzymatic antioxidants, catalase (CAT; EC 1.11.1.6) and ascorbate peroxidase (APX; EC 1.11.1.11) are representative heme enzymes meant for metabolizing stress-provoked reactive oxygen species (ROS; such as H2O2) and controlling their potential impacts on cellular metabolism and functions. CAT mainly occurs in peroxisomes and catalyzes the dismutation reaction without requiring any reductant; whereas, APX has a higher affinity for H2O2 and utilizes ascorbate (AsA) as specific electron donor for the reduction of H2O2 into H2O in organelles including chloroplasts, cytosol, mitochondria, and peroxisomes. Literature is extensive on the glutathione-associated H2O2-metabolizing systems in plants. However, discussion is meager or scattered in the literature available on the biochemical and genomic characterization as well as techniques for the assays of CAT and APX and their modulation in plants under abiotic stresses. This paper aims (a) to introduce oxidative stress-causative factors and highlights their relationship with abiotic stresses in plants; (b) to overview structure, occurrence, and significance of CAT and APX in plants;