| Title |
Carbohydrate active enzyme domains from extreme thermophiles: components of a modular toolbox for lignocellulose degradation
|
|---|---|
| Published in |
Extremophiles, November 2017
|
| DOI | 10.1007/s00792-017-0974-7 |
| Pubmed ID | |
| Authors |
Jonathan Botha, Eshchar Mizrachi, Alexander A. Myburg, Don A. Cowan |
| Abstract |
Lignocellulosic biomass is a promising feedstock for the manufacture of biodegradable and renewable bioproducts. However, the complex lignocellulosic polymeric structure of woody tissue is difficult to access without extensive industrial pre-treatment. Enzyme processing of partly depolymerised biomass is an established technology, and there is evidence that high temperature (extremely thermophilic) lignocellulose degrading enzymes [carbohydrate active enzymes (CAZymes)] may enhance processing efficiency. However, wild-type thermophilic CAZymes will not necessarily be functionally optimal under industrial pre-treatment conditions. With recent advances in synthetic biology, it is now potentially possible to build CAZyme constructs from individual protein domains, tailored to the conditions of specific industrial processes. In this review, we identify a 'toolbox' of thermostable CAZyme domains from extremely thermophilic organisms and highlight recent advances in CAZyme engineering which will allow for the rational design of CAZymes tailored to specific aspects of lignocellulose digestion. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 46 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 17% |
| Student > Master | 7 | 15% |
| Student > Doctoral Student | 6 | 13% |
| Student > Bachelor | 5 | 11% |
| Lecturer | 3 | 7% |
| Other | 9 | 20% |
| Unknown | 8 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 20 | 43% |
| Agricultural and Biological Sciences | 8 | 17% |
| Environmental Science | 3 | 7% |
| Immunology and Microbiology | 3 | 7% |
| Social Sciences | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 9 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2018.
All research outputs
#4,117,697
of 23,007,887 outputs
Outputs from Extremophiles
#113
of 801 outputs
Outputs of similar age
#75,965
of 330,783 outputs
Outputs of similar age from Extremophiles
#8
of 21 outputs
Altmetric has tracked 23,007,887 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 801 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,783 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.