| Title |
Transcriptome profile analysis reveals cardiotoxicity of maduramicin in primary chicken myocardial cells
|
|---|---|
| Published in |
Archives of Toxicology, November 2017
|
| DOI | 10.1007/s00204-017-2113-8 |
| Pubmed ID | |
| Authors |
Xiuge Gao, Lin Peng, Xiangchun Ruan, Xin Chen, Hui Ji, Junxiao Ma, Han Ni, Shanxiang Jiang, Dawei Guo |
| Abstract |
Maduramicin, an excellent ionophore antibiotic, is extensively used to control coccidiosis in poultry. Numerous maduramicin intoxications have been reported in farm animal and human due to its relatively narrow safety range, with necrosis or degeneration of cardiac and skeletal muscles as hallmark. To date, the mechanisms of maduramicin-induced cardiotoxicity remain unclear in chicken and other animals. Maduramicin (5 µg/mL)-treated primary chicken myocardial cells were used for RNA sequencing (RNA-Seq) and bioinformatics analysis in this study. A total of 1442 differential expressed genes were identified. 810 genes were up-regulated and the rest 632 genes were down-regulated. Transcriptome analysis revealed that the cytokine-cytokine receptor interaction, apoptosis, calcium signal and cytoplasmic vacuolization pathways were significantly affected. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis showed that gene expression patterns were consistent with RNA-Seq analysis. Pro-inflammatory cytokines including tumor necrosis factor alpha (TNF-α) and interleukin-8 (IL-8), apoptosis ratios, cleaved caspase-3, intracellular calcium level and Ca(2+)-ATPase activity were elevated after maduramicin (0.05, 0.5 and 5 µg/mL) treatment. Massive vacuole formation was found in the cytoplasm by morphology and transmission electron microscopy observation. Taken together, the results suggested that maduramicin exerted its cardiotoxicity by multiple molecular mechanisms in primary chicken myocardial cells. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 27% |
| Professor > Associate Professor | 2 | 13% |
| Student > Bachelor | 2 | 13% |
| Unknown | 7 | 47% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 2 | 13% |
| Computer Science | 2 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
| Agricultural and Biological Sciences | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 7 | 47% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 November 2017.
All research outputs
#17,919,786
of 23,007,887 outputs
Outputs from Archives of Toxicology
#2,164
of 2,652 outputs
Outputs of similar age
#233,856
of 326,841 outputs
Outputs of similar age from Archives of Toxicology
#24
of 35 outputs
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