| Title |
Characterization of incomplete vitellogenin (VgC) in the Indian freshwater murrel, Channa punctatus (Bloch)
|
|---|---|
| Published in |
Fish Physiology and Biochemistry, November 2014
|
| DOI | 10.1007/s10695-014-0009-6 |
| Pubmed ID | |
| Authors |
S. Pipil, V. S. Rawat, L. Sharma, N. Sehgal |
| Abstract |
A novel incomplete vitellogenin (VgC) was purified from the plasma of estradiol-treated male murrel, Channa punctatus, by gel filtration chromatography. The native mass of VgC protein was 180 kDa, and it resolved as a single peptide of 100 kDa on SDS-PAGE. The peptide on subjecting to matrix-assisted laser desorption/ionization-time of flight produced a peptide mass fingerprint. On tandem mass spectrometry, some of these peptides showed mass to charge (m/z) ratio and amino acid sequence similarity with VgC peptides of other teleosts. Phylogenetic analysis revealed a similarity of murrel VgC with fish species of the order Perciformes. Semi-quantitative RT-PCR assay was developed to study expression of vgc gene at variable levels of estradiol exposure. Presence of VgC in males indicates that fish has been exposed to estrogens; hence, it can be used as a biomarker for estrogenic exposure. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 27% |
| Researcher | 2 | 18% |
| Student > Postgraduate | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 4 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 36% |
| Veterinary Science and Veterinary Medicine | 1 | 9% |
| Biochemistry, Genetics and Molecular Biology | 1 | 9% |
| Chemistry | 1 | 9% |
| Unknown | 4 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2014.
All research outputs
#15,310,081
of 22,770,070 outputs
Outputs from Fish Physiology and Biochemistry
#246
of 859 outputs
Outputs of similar age
#150,834
of 258,738 outputs
Outputs of similar age from Fish Physiology and Biochemistry
#6
of 14 outputs
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So far Altmetric has tracked 859 research outputs from this source. They receive a mean Attention Score of 1.6. This one has gotten more attention than average, scoring higher than 61% of its peers.
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We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.