| Title |
MALDI imaging in human skin tissue sections: focus on various matrices and enzymes
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, November 2012
|
| DOI | 10.1007/s00216-012-6508-3 |
| Pubmed ID | |
| Authors |
Bernd Enthaler, Maria Trusch, Markus Fischer, Claudius Rapp, Julia K. Pruns, Jens-Peter Vietzke |
| Abstract |
Matrix-assisted laser/desorption ionization (MALDI) mass-spectrometric imaging (MSI), also known as MALDI imaging, is a powerful technique for mapping biological molecules such as endogenous proteins and peptides in human skin tissue sections. A few groups have endeavored to apply MALDI-MSI to the field of skin research; however, a comprehensive article dealing with skin tissue sections and the application of various matrices and enzymes is not available. Our aim is to present a multiplex method, based on MALDI-MSI, to obtain the maximum information from skin tissue sections. Various matrices were applied to skin tissue sections: (1) 9-aminoacridine for imaging metabolites in negative ion mode; (2) sinapinic acid to obtain protein distributions; (3) α-cyano-4-hydroxycinnamic acid subsequent to on-tissue enzymatic digestion by trypsin, elastase, and pepsin, respectively, to localize the resulting peptides. Notably, substantial amounts of data were generated from the distributions retrieved for all matrices applied. Several primary metabolites, e.g. ATP, were localized and subsequently identified by on-tissue postsource decay measurements. Furthermore, maps of proteins and peptides derived from on-tissue digests were generated. Identification of peptides was achieved by elution with different solvents, mixing with α-cyano-4-hydroxycinnamic acid, and subsequent tandem mass spectrometry (MS/MS) measurements, thereby avoiding on-tissue MS/MS measurements. Highly abundant peptides were identified, allowing their use as internal calibrants in future MALDI-MSI analyses of human skin tissue sections. Elastin as an endogenous skin protein was identified only by use of elastase, showing the high potential of alternative enzymes. The results show the versatility of MALDI-MSI in the field of skin research. This article containing a methodological perspective depicts the basics for a comprehensive comparison of various skin states. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 1% |
| China | 1 | 1% |
| Germany | 1 | 1% |
| Switzerland | 1 | 1% |
| Unknown | 75 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 18% |
| Researcher | 14 | 18% |
| Student > Master | 13 | 16% |
| Student > Bachelor | 8 | 10% |
| Student > Doctoral Student | 7 | 9% |
| Other | 14 | 18% |
| Unknown | 9 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 23 | 29% |
| Biochemistry, Genetics and Molecular Biology | 14 | 18% |
| Agricultural and Biological Sciences | 14 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 8% |
| Medicine and Dentistry | 3 | 4% |
| Other | 8 | 10% |
| Unknown | 11 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 February 2015.
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#8,261,756
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Outputs from Analytical & Bioanalytical Chemistry
#1,975
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Outputs of similar age
#63,077
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#19
of 67 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 9,619 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done well, scoring higher than 77% of its peers.
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We're also able to compare this research output to 67 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.