↓ Skip to main content

Thrombospondin Type-1 Domain-Containing 7A in Idiopathic Membranous Nephropathy

Overview of attention for article published in New England Journal of Medicine, November 2014
Altmetric Badge

About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (98th percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

Citations

dimensions_citation
754 Dimensions

Readers on

mendeley
294 Mendeley
citeulike
1 CiteULike
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Thrombospondin Type-1 Domain-Containing 7A in Idiopathic Membranous Nephropathy
Published in
New England Journal of Medicine, November 2014
DOI 10.1056/nejmoa1409354
Pubmed ID
Authors

Nicola M Tomas, Laurence H Beck, Catherine Meyer-Schwesinger, Barbara Seitz-Polski, Hong Ma, Gunther Zahner, Guillaume Dolla, Elion Hoxha, Udo Helmchen, Anne-Sophie Dabert-Gay, Delphine Debayle, Michael Merchant, Jon Klein, David J Salant, Rolf A K Stahl, Gérard Lambeau

Abstract

Background Idiopathic membranous nephropathy is an autoimmune disease. In approximately 70% of patients, it is associated with autoantibodies against the phospholipase A2 receptor 1 (PLA2R1). Antigenic targets in the remaining patients are unknown. Methods Using Western blotting, we screened serum samples from patients with idiopathic membranous nephropathy, patients with other glomerular diseases, and healthy controls for antibodies against human native glomerular proteins. We partially purified a putative new antigen, identified this protein by means of mass spectrometry of digested peptides, and validated the results by analysis of recombinant protein expression, immunoprecipitation, and immunohistochemical analysis. Results Serum samples from 6 of 44 patients in a European cohort and 9 of 110 patients in a Boston cohort with anti-PLA2R1-negative idiopathic membranous nephropathy recognized a glomerular protein that was 250 kD in size. None of the serum samples from the 74 patients with idiopathic membranous nephropathy who were seropositive for anti-PLA2R1 antibodies, from the 76 patients with other glomerular diseases, and from the 44 healthy controls reacted against this antigen. Although this newly identified antigen is clearly different from PLA2R1, it shares some biochemical features, such as N-glycosylation, membranous location, and reactivity with serum only under nonreducing conditions. Mass spectrometry identified this antigen as thrombospondin type-1 domain-containing 7A (THSD7A). All reactive serum samples recognized recombinant THSD7A and immunoprecipitated THSD7A from glomerular lysates. Moreover, immunohistochemical analyses of biopsy samples from patients revealed localization of THSD7A to podocytes, and IgG eluted from one of these samples was specific for THSD7A. Conclusions In our cohort, 15 of 154 patients with idiopathic membranous nephropathy had circulating autoantibodies to THSD7A but not to PLA2R1, a finding that suggests a distinct subgroup of patients with this condition. (Funded by the French National Center for Scientific Research and others.).

X Demographics

X Demographics

The data shown below were collected from the profiles of 14 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 294 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 <1%
France 1 <1%
Unknown 292 99%

Demographic breakdown

Readers by professional status Count As %
Researcher 46 16%
Other 31 11%
Student > Ph. D. Student 24 8%
Student > Master 24 8%
Student > Bachelor 23 8%
Other 60 20%
Unknown 86 29%
Readers by discipline Count As %
Medicine and Dentistry 139 47%
Biochemistry, Genetics and Molecular Biology 23 8%
Agricultural and Biological Sciences 14 5%
Immunology and Microbiology 7 2%
Engineering 3 1%
Other 15 5%
Unknown 93 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 96. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 November 2023.
All research outputs
#441,254
of 25,374,647 outputs
Outputs from New England Journal of Medicine
#6,188
of 32,473 outputs
Outputs of similar age
#4,560
of 270,396 outputs
Outputs of similar age from New England Journal of Medicine
#88
of 309 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 32,473 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 122.0. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 270,396 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 309 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.