| Title |
Clonal in vitro propagation of peat mosses (Sphagnum L.) as novel green resources for basic and applied research
|
|---|---|
| Published in |
Plant Cell, Tissue and Organ Culture (PCTOC), November 2014
|
| DOI | 10.1007/s11240-014-0658-2 |
| Pubmed ID | |
| Authors |
Anna K. Beike, Valeria Spagnuolo, Volker Lüth, Feray Steinhart, Julia Ramos-Gómez, Matthias Krebs, Paola Adamo, Ana Isabel Rey-Asensio, J. Angel Fernández, Simonetta Giordano, Eva L. Decker, Ralf Reski |
| Abstract |
As builders and major components of peatlands, Sphagnopsida (peat mosses) are very important organisms for ecosystems and world's climate. Nowadays many Sphagnum species as well as their habitats are largely protected, while their scientific and economic relevance remains considerable. Advanced methods of in vitro cultivation provide the potential to work in a sustainable way with peat mosses and address aspects of basic research as well as biotechnological and economical topics like biomonitoring or the production of renewable substrates for horticulture (Sphagnum farming). Here, we describe the establishment of axenic in vitro cultures of the five peat moss species Sphagnum fimbriatum Wils. and Hook., Sphagnum magellanicum Brid., Sphagnum palustre L., Sphagnum rubellum Wils. and Sphagnum subnitens Russ. and Warnst. with specific focus on large-scale cultivation of S. palustre in bioreactors. Axenic, clonal cultures were established to produce high quantities of biomass under standardized laboratory conditions. For advanced production of S. palustre we tested different cultivation techniques, growth media and inocula, and analyzed the effects of tissue disruption. While cultivation on solid medium is suitable for long term storage, submerse cultivation in liquid medium yielded highest amounts of biomass. By addition of sucrose and ammonium nitrate we were able to increase the biomass by around 10- to 30-fold within 4 weeks. The morphology of in vitro-cultivated gametophores showed similar phenotypic characteristics compared to material from the field. Thus the tested culture techniques are suitable to produce S. palustre material for basic and applied research. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 127 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 2% |
| Netherlands | 1 | <1% |
| Chile | 1 | <1% |
| Unknown | 123 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 21 | 17% |
| Researcher | 20 | 16% |
| Student > Ph. D. Student | 18 | 14% |
| Student > Bachelor | 12 | 9% |
| Student > Doctoral Student | 8 | 6% |
| Other | 24 | 19% |
| Unknown | 24 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 46 | 36% |
| Biochemistry, Genetics and Molecular Biology | 18 | 14% |
| Environmental Science | 15 | 12% |
| Chemistry | 4 | 3% |
| Unspecified | 3 | 2% |
| Other | 13 | 10% |
| Unknown | 28 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2021.
All research outputs
#3,303,413
of 23,911,072 outputs
Outputs from Plant Cell, Tissue and Organ Culture (PCTOC)
#34
of 1,060 outputs
Outputs of similar age
#38,028
of 261,504 outputs
Outputs of similar age from Plant Cell, Tissue and Organ Culture (PCTOC)
#1
of 14 outputs
Altmetric has tracked 23,911,072 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,060 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 261,504 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.