| Title |
Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011–2013) reveals rapid B602L central variable region evolution
|
|---|---|
| Published in |
Virus Genes, November 2017
|
| DOI | 10.1007/s11262-017-1521-4 |
| Pubmed ID | |
| Authors |
C. K. Onzere, A. D. Bastos, E. A. Okoth, J. K. Lichoti, E. N. Bochere, M. G. Owido, G. Ndambuki, M. Bronsvoort, R. P. Bishop |
| Abstract |
The central variable region (CVR) within the B602L gene of the African swine fever virus (ASFV) is highly polymorphic within the 23 ASFV genotypes defined by sequencing of the C-terminal end of the p72 locus. Sequencing the p54 gene further discriminates ASFV genotypes that are conserved at the p72 locus. Variation in the thymidine kinase locus is a novel additional tool for ASFV genotyping whose application for this purpose is described for the first time herein. We evaluated genetic variation at these four polymorphic loci in 39 ASFV isolates obtained from outbreaks in Kenya and a region of Eastern Uganda between 2011 and 2013. Analysis of the p72 and p54 loci revealed high genetic conservation among these isolates; all clustered within p72 genotype IX and were similar to isolates associated with earlier outbreaks in East Africa. The thymidine kinase gene of the Kenyan isolates in this study were distinct relative to Southern African isolates and synonymous substitutions were observed among viruses from central Kenya. Analysis of the CVR within the B602L gene revealed two previously unknown polymorphisms that were restricted to Western Kenya and Eastern Uganda. A novel variant was revealed within CVR subgroup XXIV and a novel CVR subgroup XXIVa that contains tetrameric repeat F which has previously only been associated with p72 genotype I, was also identified for the first time in East Africa. Phylogeographic analysis of isolates based on CVR polymorphisms revealed rapid evolution and dissemination of variants present within ASFV genotype IX in East Africa. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 17% |
| Canada | 1 | 17% |
| Unknown | 4 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 83% |
| Scientists | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 57 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 19% |
| Researcher | 9 | 16% |
| Student > Doctoral Student | 5 | 9% |
| Student > Bachelor | 4 | 7% |
| Other | 4 | 7% |
| Other | 10 | 18% |
| Unknown | 14 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 12 | 21% |
| Agricultural and Biological Sciences | 8 | 14% |
| Biochemistry, Genetics and Molecular Biology | 7 | 12% |
| Medicine and Dentistry | 3 | 5% |
| Immunology and Microbiology | 2 | 4% |
| Other | 6 | 11% |
| Unknown | 19 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 September 2019.
All research outputs
#7,317,536
of 23,314,015 outputs
Outputs from Virus Genes
#171
of 985 outputs
Outputs of similar age
#117,487
of 325,820 outputs
Outputs of similar age from Virus Genes
#3
of 13 outputs
Altmetric has tracked 23,314,015 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 985 research outputs from this source. They receive a mean Attention Score of 2.4. This one has done well, scoring higher than 82% of its peers.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.