| Chapter title |
Synthesis of genetically engineered protein polymers (recombinamers) as an example of advanced self-assembled smart materials.
|
|---|---|
| Chapter number | 2 |
| Book title |
Nanotechnology in Regenerative Medicine
|
| Published in |
Methods in molecular biology, November 2011
|
| DOI | 10.1007/978-1-61779-388-2_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-387-5, 978-1-61779-388-2
|
| Authors |
Rodríguez-Cabello JC, Girotti A, Ribeiro A, Arias FJ, José Carlos Rodríguez-Cabello, Alessandra Girotti, Artur Ribeiro, Francisco Javier Arias, Rodríguez-Cabello, José Carlos, Girotti, Alessandra, Ribeiro, Artur, Arias, Francisco Javier |
| Abstract |
In this chapter, we describe two methods for bio-producing recombinant repetitive polypeptide polymers for use in biomedical devices. These polymers, known as elastin-like recombinamers (ELRs), are derived from the repetition of selected amino acid domains of extracellular matrix proteins with the aim of recreating their mechanical and physiological features. The proteinaceous nature of ELRs allows us to make use of the natural biosynthetic machinery of heterologous hosts to express advanced and large polymers or "recombinamers." Despite the essentially unlimited possibilities for designing recombinamers, the production of synthetic genes to encode them should allow us to overcome the difficulties surrounding bioproduction of these non-natural monotonous DNA and protein sequences. The aim of this work is to supply the biotechnologist with fine-tuning methods to biosynthesize advanced self-assembled smart materials. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 2% |
| Unknown | 60 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 20% |
| Student > Ph. D. Student | 10 | 16% |
| Student > Bachelor | 6 | 10% |
| Student > Master | 6 | 10% |
| Student > Postgraduate | 3 | 5% |
| Other | 5 | 8% |
| Unknown | 19 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 20% |
| Materials Science | 6 | 10% |
| Chemistry | 4 | 7% |
| Agricultural and Biological Sciences | 3 | 5% |
| Engineering | 3 | 5% |
| Other | 12 | 20% |
| Unknown | 21 | 34% |