| Title |
Enzymatic cross-linking of human recombinant elastin (HELP) as biomimetic approach in vascular tissue engineering
|
|---|---|
| Published in |
Journal of Materials Science: Materials in Medicine, October 2011
|
| DOI | 10.1007/s10856-011-4451-z |
| Pubmed ID | |
| Authors |
Sabrina Bozzini, Liliana Giuliano, Lina Altomare, Paola Petrini, Antonella Bandiera, Maria Teresa Conconi, Silvia Farè, Maria Cristina Tanzi |
| Abstract |
The use of polymers naturally occurring in the extracellular matrix (ECM) is a promising strategy in regenerative medicine. If compared to natural ECM proteins, proteins obtained by recombinant DNA technology have intrinsic advantages including reproducible macromolecular composition, sequence and molecular mass, and overcoming the potential pathogens transmission related to polymers of animal origin. Among ECM-mimicking materials, the family of recombinant elastin-like polymers is proposed for drug delivery applications and for the repair of damaged elastic tissues. This work aims to evaluate the potentiality of a recombinant human elastin-like polypeptide (HELP) as a base material of cross-linked matrices for regenerative medicine. The cross-linking of HELP was accomplished by the insertion of cross-linking sites, glutamine and lysine, in the recombinant polymer and generating ε-(γ-glutamyl) lysine links through the enzyme transglutaminase. The cross-linking efficacy was estimated by infrared spectroscopy. Freeze-dried cross-linked matrices showed swelling ratios in deionized water (≈2500%) with good structural stability up to 24 h. Mechanical compression tests, performed at 37°C in wet conditions, in a frequency sweep mode, indicated a storage modulus of 2/3 kPa, with no significant changes when increasing number of cycles or frequency. These results demonstrate the possibility to obtain mechanically resistant hydrogels via enzymatic crosslinking of HELP. Cytotoxicity tests of cross-linked HELP were performed with human umbilical vein endothelial cells, by use of transwell filter chambers for 1-7 days, or with its extracts in the opportune culture medium for 24 h. In both cases no cytotoxic effects were observed in comparison with the control cultures. On the whole, the results suggest the potentiality of this genetically engineered HELP for regenerative medicine applications, particularly for vascular tissue regeneration. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Italy | 1 | 2% |
| Unknown | 49 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 29% |
| Student > Master | 10 | 20% |
| Researcher | 7 | 14% |
| Professor > Associate Professor | 4 | 8% |
| Student > Doctoral Student | 3 | 6% |
| Other | 7 | 14% |
| Unknown | 5 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Materials Science | 7 | 14% |
| Agricultural and Biological Sciences | 7 | 14% |
| Medicine and Dentistry | 7 | 14% |
| Engineering | 6 | 12% |
| Biochemistry, Genetics and Molecular Biology | 5 | 10% |
| Other | 7 | 14% |
| Unknown | 12 | 24% |
Attention Score in Context
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Outputs from Journal of Materials Science: Materials in Medicine
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Outputs of similar age from Journal of Materials Science: Materials in Medicine
#17
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