| Title |
Down-regulation of testes-specific protease 50 induces apoptosis in human laryngocarcinoma HEp2 cells in a NF-κB-mediated pathway
|
|---|---|
| Published in |
Molecular Biology Reports, November 2014
|
| DOI | 10.1007/s11033-014-3634-3 |
| Pubmed ID | |
| Authors |
Yu-lin Liu, Ya-nan Sun |
| Abstract |
Testes-specific protease 50 is a newly reported threonine enzyme. It has similar amino acid sequences and enzymatic structures to some other serine proteases. It is proposed as a laryngocarcinoma-related gene in human beings. The physiological mechanism by which TSP50 exerts its promoting effects in laryngocarcinoma is not yet fully understood. The study investigated the function of TSP50 by suppressing its expression in the HEp2 cell line using a TSP50-specific short hairpin RNA (shRNA). Western bloting and real-time-PCR were used to detect the levels of TSP50. By using MTT, Wound healing, flow cytometric and tumorigenesis assays, the study tested the TSP50 role in human laryngocarcinoma cell growth and apoptosis. The results demonstrated that TSP50 knockdown could inhibit HEp2 cell proliferation and induce apoptosis in vitro in a NF-κB-mediated pathway. The tumorigenicity of TSP50 shRNA-expressing cells were decreased after inoculating into nude mice. The present results provide a new understanding of the TSP50 gene in the progression of laryngocarcinoma and put up a novel therapeutic target for treating this cancer. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 67% |
| Student > Master | 1 | 17% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 5 | 83% |
| Unknown | 1 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 January 2016.
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#18,383,471
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#1,601
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#165,849
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Outputs of similar age from Molecular Biology Reports
#19
of 35 outputs
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