Title |
Massively parallel sequencing of forensically relevant single nucleotide polymorphisms using TruSeq™ forensic amplicon
|
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Published in |
International Journal of Legal Medicine, November 2014
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DOI | 10.1007/s00414-014-1108-8 |
Pubmed ID | |
Authors |
David H. Warshauer, Carey P. Davis, Cydne Holt, Yonmee Han, Paulina Walichiewicz, Tom Richardson, Kathryn Stephens, Anne Jager, Jonathan King, Bruce Budowle |
Abstract |
The TruSeq™ Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq™ ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. In this study, the TruSeq™ Forensic Amplicon library preparation protocol was used to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples. Results were compared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq™ Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS). |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 34 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 26% |
Student > Bachelor | 5 | 15% |
Student > Master | 4 | 12% |
Student > Doctoral Student | 4 | 12% |
Researcher | 4 | 12% |
Other | 3 | 9% |
Unknown | 5 | 15% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 12 | 35% |
Agricultural and Biological Sciences | 8 | 24% |
Chemistry | 2 | 6% |
Nursing and Health Professions | 1 | 3% |
Environmental Science | 1 | 3% |
Other | 2 | 6% |
Unknown | 8 | 24% |