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Plant Cell Expansion

Overview of attention for book
Cover of 'Plant Cell Expansion'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Penium margaritaceum as a Model Organism for Cell Wall Analysis of Expanding Plant Cells
  3. Altmetric Badge
    Chapter 2 Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum
  4. Altmetric Badge
    Chapter 3 Optimized Method for Growing In Vitro Arabidopsis thaliana Pollen Tubes
  5. Altmetric Badge
    Chapter 4 Imaging of Calcium Dynamics in Pollen Tube Cytoplasm
  6. Altmetric Badge
    Chapter 5 Live Imaging of Root Hairs
  7. Altmetric Badge
    Chapter 6 Improved ROS Measurement in Root Hair Cells
  8. Altmetric Badge
    Chapter 7 Plant Cell Expansion
  9. Altmetric Badge
    Chapter 8 Vacuolar Staining Methods in Plant Cells
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    Chapter 9 Live Cell Imaging of FM4-64, a Tool for Tracing the Endocytic Pathways in Arabidopsis Root Cells
  11. Altmetric Badge
    Chapter 10 Salt-Stress Regulation of Root System Growth and Architecture in Arabidopsis Seedlings.
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    Chapter 11 Quantification of Fluorescent Reporters in Plant Cells
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    Chapter 12 Live Cell Imaging of the Cytoskeleton and Cell Wall Enzymes in Plant Cells
  14. Altmetric Badge
    Chapter 13 Using the Split-Ubiquitin Yeast Two-Hybrid System to Test Protein-Protein Interactions of Transmembrane Proteins.
  15. Altmetric Badge
    Chapter 14 Activation Tag Screening for Cell Expansion Genes in Arabidopsis thaliana
  16. Altmetric Badge
    Chapter 15 BiFC for Protein–Protein Interactions and Protein Topology: Discussing an Integrative Approach for an Old Technique
  17. Altmetric Badge
    Chapter 16 N-Glycosylation and Plant Cell Growth
  18. Altmetric Badge
    Chapter 17 Peptide Separation Methodologies for In-depth Proteomics.
  19. Altmetric Badge
    Chapter 18 Structural and Mechanical Characterization of Growing Arabidopsis Plant Cell Walls
Attention for Chapter 13: Using the Split-Ubiquitin Yeast Two-Hybrid System to Test Protein-Protein Interactions of Transmembrane Proteins.
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Chapter title
Using the Split-Ubiquitin Yeast Two-Hybrid System to Test Protein-Protein Interactions of Transmembrane Proteins.
Chapter number 13
Book title
Plant Cell Expansion
Published in
Methods in molecular biology, October 2014
DOI 10.1007/978-1-4939-1902-4_13
Pubmed ID
25408451
Book ISBNs
978-1-4939-1901-7, 978-1-4939-1902-4
Authors

Logan Bashline, Ying Gu

Editors

José M. Estevez

Abstract

Proteins are responsible for many biological processes within living organisms. Many proteins have the ability to specifically interact with other proteins in order to function properly. The identification of protein-protein interactions (PPIs) can provide useful information about the function of a protein of interest. Historically, the properties of transmembrane proteins have caused difficulty in analyzing PPIs among transmembrane proteins. The development of an assay that is capable of analyzing PPIs involving transmembrane proteins, the split-ubiquitin yeast two-hybrid (SU-Y2H) assay, has provided a method to probe pairwise PPIs between two proteins of interest or to screen a single protein of interest for interaction partners. The following protocol explains how to use the SU-Y2H assay, which is compatible with the use of transmembrane proteins, to investigate PPIs between two proteins of interest and also briefly describes how to adjust the system to be used as a high-throughput screen for interaction partners of a particular protein of interest.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 29%
Student > Bachelor 3 21%
Researcher 2 14%
Student > Doctoral Student 1 7%
Unknown 4 29%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 43%
Biochemistry, Genetics and Molecular Biology 3 21%
Chemistry 1 7%
Unknown 4 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2014.
All research outputs
#20,243,777
of 22,771,140 outputs
Outputs from Methods in molecular biology
#9,862
of 13,090 outputs
Outputs of similar age
#217,266
of 260,458 outputs
Outputs of similar age from Methods in molecular biology
#97
of 133 outputs
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So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 133 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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