Title |
HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood
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Published in |
Vaccine, May 2005
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DOI | 10.1016/j.vaccine.2005.01.146 |
Pubmed ID | |
Authors |
Ligia A. Pinto, Philip E. Castle, Richard B. Roden, Clayton D. Harro, Douglas R. Lowy, John T. Schiller, Dora Wallace, Marcus Williams, William Kopp, Ian H. Frazer, Jay A. Berzofsky, Allan Hildesheim |
Abstract |
Here, we evaluated innate and adaptive immune system cytokine responses induced by HPV-16 L1 VLP in whole blood (WB) cultures from individuals receiving the vaccine (n=20) or placebo (n=4) before and after vaccination. 11 cytokines were measured: IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IFN-gamma, TNF-alpha, and GM-CSF using multiplex bead arrays. Cytokine profiles from WB samples clearly discriminated between vaccine and placebo recipients and between pre and post-vaccination responses. Significant increases in Th1, Th2 and inflammatory cytokines were observed in WB assays following vaccination. Results from WB assays were compared against parallel PBMC-based assays in a subset of patients. Differences between whole blood assay and PBMC were observed, with the highest levels of induction found for WB for several cytokines. Our results indicate that multiplex assays for cytokine profiling in WB are an efficient tool for assessing broad spectrum, innate and adaptive immune responses to vaccines and identifying immunologic correlates of protection in efficacy studies. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 2% |
Argentina | 1 | 2% |
Unknown | 48 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 12 | 24% |
Student > Ph. D. Student | 8 | 16% |
Student > Bachelor | 5 | 10% |
Other | 5 | 10% |
Student > Master | 5 | 10% |
Other | 8 | 16% |
Unknown | 7 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 12 | 24% |
Agricultural and Biological Sciences | 9 | 18% |
Biochemistry, Genetics and Molecular Biology | 6 | 12% |
Immunology and Microbiology | 5 | 10% |
Nursing and Health Professions | 1 | 2% |
Other | 6 | 12% |
Unknown | 11 | 22% |