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Attention Score in Context
| Title |
Assessing Steatotic Liver Function after Ischemia-Reperfusion Injury by In Vivo Multiphoton Imaging of Fluorescein Disposition
|
|---|---|
| Published in |
Drug Metabolism and Disposition, November 2014
|
| DOI | 10.1124/dmd.114.060848 |
| Pubmed ID | |
| Authors |
Camilla A. Thorling, Lu Jin, Michael Weiss, Darrell Crawford, Xin Liu, Frank J. Burczynski, David Liu, Haolu Wang, Michael S. Roberts |
| Abstract |
Ischemia-reperfusion injury is a common complication during liver surgery, where steatotic livers are more prone to the injury and may become more prevalent in the growing obese population. This study aimed to characterize liver morphology and understand changes in subcellular function in steatotic livers exposed to ischemia-reperfusion injury through quantitative description of fluorescein distribution obtained by minimally-invasive in vivo multiphoton microscopy using a physiological pharmacokinetic model. Rats were fed a high fat diet for 7 days to induce liver steatosis. Partial ischemia was induced, following reperfusion for 4 hours, when fluorescein (10 mg/kg) was injected intravenously. Liver images, bile and blood were collected up to 180 min following injection. Ischemia-reperfusion injury was associated with an increase in alanine transaminase levels and apoptosis. In addition, steatosis had the presence of lipid droplets and an increase in the fluorescein associated fluorescence observed in the hepatocytes by multiphoton imaging. Analysis of the hepatic concentration-time profiles suggests that the steatosis induced increase in fluorescein associated fluorescence mainly arises by inducing the hepatic fluorescein metabolism. The combination of ischemia-reperfusion with steatosis exacerbates these effects further. This was confirmed by fluorescence lifetime imaging microscopy showing a decreased average fluorescence lifetime of the liver, indicative of increased production of the metabolite. Our results show the potential of non-invasive imaging of a dye to further improve our understanding of liver disease induced subcellular changes in vivo by also providing further quantitative measures of metabolic and biliary liver function and, hence, extending the qualitative liver function tests now available. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 27% |
| Student > Master | 3 | 20% |
| Researcher | 2 | 13% |
| Student > Bachelor | 1 | 7% |
| Student > Doctoral Student | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 3 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 27% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 13% |
| Physics and Astronomy | 2 | 13% |
| Agricultural and Biological Sciences | 1 | 7% |
| Unspecified | 1 | 7% |
| Other | 2 | 13% |
| Unknown | 3 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 November 2014.
All research outputs
#20,243,777
of 22,771,140 outputs
Outputs from Drug Metabolism and Disposition
#2,189
of 2,306 outputs
Outputs of similar age
#219,364
of 262,839 outputs
Outputs of similar age from Drug Metabolism and Disposition
#12
of 20 outputs
Altmetric has tracked 22,771,140 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,306 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,839 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.