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Ion-pair high-performance liquid chromatographic assay method for the assessment of clarithromycin stability in aqueous solution and in gastric juice

Overview of attention for article published in Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences, June 1996
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Title
Ion-pair high-performance liquid chromatographic assay method for the assessment of clarithromycin stability in aqueous solution and in gastric juice
Published in
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences, June 1996
DOI 10.1016/0378-4347(96)00064-3
Pubmed ID
Authors

P.O. Erah, D.A. Barrett, P.N. Shaw

Abstract

A simple and selective ion-pair HPLC method has been developed for the analysis of clarithromycin in aqueous solutions and in gastric juice. A Hypersil ODS 5-microns (150 x 4.6 mm I.D.) column was used with a mobile phase consisting of acetonitrile-aqueous 0.05 M phosphate buffer (pH 4.6) containing 5 mM l-octanesulphonic acid (50:50, v/v). The column temperature was 50 degrees C and detection was by UV absorption (210 nm). The limits of detection of 50-microliters samples were 0.4 microgram/ml (aqueous) and 0.78 microgram/ml (0.5 ml gastric juice) or better. The assay was linear in the range of 1.56 to 100 micrograms/ml with r2 values greater than 0.99. The recovery from the gastric juice samples was 98.5 +/- 2.9%. The method was applied successfully to determine the stability of clarithromycin in 0.01 M HCl and gastric juice.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Slovenia 1 6%
Unknown 16 94%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 18%
Lecturer 2 12%
Student > Bachelor 2 12%
Professor 2 12%
Researcher 2 12%
Other 3 18%
Unknown 3 18%
Readers by discipline Count As %
Medicine and Dentistry 3 18%
Pharmacology, Toxicology and Pharmaceutical Science 2 12%
Nursing and Health Professions 1 6%
Arts and Humanities 1 6%
Physics and Astronomy 1 6%
Other 3 18%
Unknown 6 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2014.
All research outputs
#22,758,309
of 25,373,627 outputs
Outputs from Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
#4,321
of 5,117 outputs
Outputs of similar age
#26,044
of 26,524 outputs
Outputs of similar age from Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
#31
of 31 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,117 research outputs from this source. They receive a mean Attention Score of 3.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.