| Title |
Impact of prolonged overfeeding on skeletal muscle mitochondria in healthy individuals
|
|---|---|
| Published in |
Diabetologia, November 2017
|
| DOI | 10.1007/s00125-017-4496-8 |
| Pubmed ID | |
| Authors |
Frederico G. S. Toledo, Darcy L. Johannsen, Jeffrey D. Covington, Sudip Bajpeyi, Bret Goodpaster, Kevin E. Conley, Eric Ravussin |
| Abstract |
Reduced mitochondrial capacity in skeletal muscle has been observed in obesity and type 2 diabetes. In humans, the aetiology of this abnormality is not well understood but the possibility that it is secondary to the stress of nutrient overload has been suggested. To test this hypothesis, we examined whether sustained overfeeding decreases skeletal muscle mitochondrial content or impairs function. Twenty-six healthy volunteers (21 men, 5 women, age 25.3 ± 4.5 years, BMI 25.5 ± 2.4 kg/m(2)) underwent a supervised protocol consisting of 8 weeks of high-fat overfeeding (40% over baseline energy requirements). Before and after overfeeding, we measured systemic fuel oxidation by indirect calorimetry and performed skeletal muscle biopsies to measure mitochondrial gene expression, content and function in vitro. Mitochondrial function in vivo was measured by (31)P NMR spectroscopy. With overfeeding, volunteers gained 7.7 ± 1.8 kg (% change 9.8 ± 2.3). Overfeeding increased fasting NEFA, LDL-cholesterol and insulin concentrations. Indirect calorimetry showed a shift towards greater reliance on lipid oxidation. In skeletal muscle tissue, overfeeding increased ceramide content, lipid droplet content and perilipin-2 mRNA expression. Phosphorylation of AMP-activated protein kinase was decreased. Overfeeding increased mRNA expression of certain genes coding for mitochondrial proteins (CS, OGDH, CPT1B, UCP3, ANT1). Despite the stress of nutrient overload, mitochondrial content and mitochondrial respiration in muscle did not change after overfeeding. Similarly, overfeeding had no effect on either the emission of reactive oxygen species or on mitochondrial function in vivo. Skeletal muscle mitochondria are significantly resilient to nutrient overload. The lower skeletal muscle mitochondrial oxidative capacity in human obesity is likely to be caused by reasons other than nutrient overload per se. ClinicalTrials.gov NCT01672632. |
X Demographics
The data shown below were collected from the profiles of 71 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 16 | 23% |
| United Kingdom | 11 | 15% |
| Canada | 4 | 6% |
| Chile | 3 | 4% |
| Germany | 3 | 4% |
| Australia | 2 | 3% |
| Spain | 2 | 3% |
| New Zealand | 2 | 3% |
| Argentina | 1 | 1% |
| Other | 6 | 8% |
| Unknown | 21 | 30% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 38 | 54% |
| Scientists | 20 | 28% |
| Practitioners (doctors, other healthcare professionals) | 13 | 18% |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 69 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 15 | 22% |
| Researcher | 8 | 12% |
| Student > Ph. D. Student | 7 | 10% |
| Student > Bachelor | 6 | 9% |
| Other | 5 | 7% |
| Other | 9 | 13% |
| Unknown | 19 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 14 | 20% |
| Agricultural and Biological Sciences | 10 | 14% |
| Biochemistry, Genetics and Molecular Biology | 5 | 7% |
| Sports and Recreations | 5 | 7% |
| Nursing and Health Professions | 4 | 6% |
| Other | 2 | 3% |
| Unknown | 29 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 61. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2021.
All research outputs
#659,557
of 24,338,161 outputs
Outputs from Diabetologia
#351
of 5,249 outputs
Outputs of similar age
#15,109
of 440,080 outputs
Outputs of similar age from Diabetologia
#11
of 67 outputs
Altmetric has tracked 24,338,161 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,249 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 24.0. This one has done particularly well, scoring higher than 93% of its peers.
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We're also able to compare this research output to 67 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.