Title |
Limited niche availability suppresses murine intrathymic dendritic-cell development from noncommitted progenitors
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Published in |
Blood, November 2014
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DOI | 10.1182/blood-2014-07-592667 |
Pubmed ID | |
Authors |
Marcin Łyszkiewicz, Natalia Ziętara, Lisa Föhse, Jacek Puchałka, Jana Diestelhorst, Katrin Witzlau, Immo Prinz, Axel Schambach, Andreas Krueger |
Abstract |
The origins of dendritic cells (DCs) and other myeloid cells in the thymus have remained controversial. Here, we assessed developmental relationships between thymic (t)DCs and thymocytes employing retrovirus-based cellular barcoding, reporter mice as well as intrathymic transfers coupled with DC depletion. We demonstrated that a subset of early T lineage progenitors expressed the bona-fide marker for DC progenitors, CX3CR1. However, intrathymic transfers into non-manipulated mice as well as retroviral barcoding indicated that tDCs and thymocytes were largely of distinct developmental origin. In contrast, intrathymic transfers after in vivo depletion of DC resulted in intrathymic development of non-T lineage cells. In conclusion, our data support a model in which adoption of T lineage fate by non-committed progenitors at steady state is enforced by signals from the thymic microenvironment unless niches promoting alternative lineage fates become available. |
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