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Attention Score in Context
| Title |
Centromere sliding on a mammalian chromosome
|
|---|---|
| Published in |
Chromosoma, November 2014
|
| DOI | 10.1007/s00412-014-0493-6 |
| Pubmed ID | |
| Authors |
Stefania Purgato, Elisa Belloni, Francesca M. Piras, Monica Zoli, Claudia Badiale, Federico Cerutti, Alice Mazzagatti, Giovanni Perini, Giuliano Della Valle, Solomon G. Nergadze, Kevin F. Sullivan, Elena Raimondi, Mariano Rocchi, Elena Giulotto |
| Abstract |
The centromere directs the segregation of chromosomes during mitosis and meiosis. It is a distinct genetic locus whose identity is established through epigenetic mechanisms that depend on the deposition of centromere-specific centromere protein A (CENP-A) nucleosomes. This important chromatin domain has so far escaped comprehensive molecular analysis due to its typical association with highly repetitive satellite DNA. In previous work, we discovered that the centromere of horse chromosome 11 is completely devoid of satellite DNA; this peculiar feature makes it a unique model to dissect the molecular architecture of mammalian centromeres. Here, we exploited this native satellite-free centromere to determine the precise localization of its functional domains in five individuals: We hybridized DNA purified from chromatin immunoprecipitated with an anti CENP-A antibody to a high resolution array (ChIP-on-chip) of the region containing the primary constriction of horse chromosome 11. Strikingly, each individual exhibited a different arrangement of CENP-A binding domains. We then analysed the organization of each domain using a single nucleotide polymorphism (SNP)-based approach and single molecule analysis on chromatin fibres. Examination of the ten instances of chromosome 11 in the five individuals revealed seven distinct 'positional alleles', each one extending for about 80-160 kb, were found across a region of about 500 kb. Our results demonstrate that CENP-A binding domains are autonomous relative to the underlying DNA sequence and are characterized by positional instability causing the sliding of centromere position. We propose that this dynamic behaviour may be common in mammalian centromeres and may determine the establishment of epigenetic alleles. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Czechia | 2 | 3% |
| United States | 1 | 2% |
| Unknown | 63 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 19 | 29% |
| Student > Ph. D. Student | 14 | 21% |
| Student > Doctoral Student | 4 | 6% |
| Professor | 4 | 6% |
| Other | 4 | 6% |
| Other | 13 | 20% |
| Unknown | 8 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 29 | 44% |
| Agricultural and Biological Sciences | 27 | 41% |
| Veterinary Science and Veterinary Medicine | 1 | 2% |
| Unknown | 9 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 November 2014.
All research outputs
#20,243,777
of 22,771,140 outputs
Outputs from Chromosoma
#695
of 757 outputs
Outputs of similar age
#302,869
of 361,837 outputs
Outputs of similar age from Chromosoma
#11
of 11 outputs
Altmetric has tracked 22,771,140 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 757 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.