| Title |
Best practices for cryopreserving, thawing, recovering, and assessing cells
|
|---|---|
| Published in |
In Vitro Cellular & Developmental Biology - Animal, November 2017
|
| DOI | 10.1007/s11626-017-0201-y |
| Pubmed ID | |
| Authors |
John M. Baust, Lia H. Campbell, John W. Harbell |
| Abstract |
Long-term storage of cell stocks insures that cells are available for use whenever needed. Cryopreservation of cells is the method of choice for preservation of important or rare cell stocks. There are several factors to consider when establishing a protocol for freezing, thawing, and recovery of cells after storage. These parameters may include cell concentration, cryoprotectant choice and concentration, and thawing rate among others. Further, the assessment of cell viability and/or function prior to and following cryopreservation is imperative in order to accurately determine downstream utility as well for optimizing the cryopreservation process. This chapter is designed to provide guidance and insight into developing robust and successful protocols for preserving cells that will preserve cell stocks and provide optimal cell yield and viability. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 33% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 167 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 167 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 23 | 14% |
| Student > Bachelor | 21 | 13% |
| Student > Ph. D. Student | 20 | 12% |
| Student > Master | 17 | 10% |
| Unspecified | 10 | 6% |
| Other | 19 | 11% |
| Unknown | 57 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 27 | 16% |
| Agricultural and Biological Sciences | 15 | 9% |
| Medicine and Dentistry | 13 | 8% |
| Unspecified | 10 | 6% |
| Engineering | 10 | 6% |
| Other | 26 | 16% |
| Unknown | 66 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2023.
All research outputs
#5,729,542
of 23,072,295 outputs
Outputs from In Vitro Cellular & Developmental Biology - Animal
#121
of 797 outputs
Outputs of similar age
#93,046
of 329,368 outputs
Outputs of similar age from In Vitro Cellular & Developmental Biology - Animal
#2
of 10 outputs
Altmetric has tracked 23,072,295 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 797 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 329,368 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 8 of them.