| Title |
Multidrug resistance-associated protein 4 is a bile transporter of Clonorchis sinensis simulated by in silico docking
|
|---|---|
| Published in |
Parasites & Vectors, November 2017
|
| DOI | 10.1186/s13071-017-2523-8 |
| Pubmed ID | |
| Authors |
Fuhong Dai, Won Gi Yoo, Ji-Yun Lee, Yanyan Lu, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong |
| Abstract |
Multidrug resistance-associated protein 4 (MRP4) is a member of the C subfamily of the ABC family of ATP-binding cassette (ABC) transporters. MRP4 regulates ATP-dependent efflux of various organic anionic substrates and bile acids out of cells. Since Clonorchis sinensis lives in host's bile duct, accumulation of bile juice can be toxic to the worm's tissues and cells. Therefore, C. sinensis needs bile transporters to reduce accumulation of bile acids within its body. We cloned MRP4 (CsMRP4) from C. sinensis and obtained a cDNA encoding an open reading frame of 1469 amino acids. Phylogenetic analysis revealed that CsMRP4 belonged to the MRP/SUR/CFTR subfamily. A tertiary structure of CsMRP4 was generated by homology modeling based on multiple structures of MRP1 and P-glycoprotein. CsMRP4 had two membrane-spanning domains (MSD1 & 2) and two nucleotide-binding domains (NBD1 & 2) as common structural folds. Docking simulation with nine bile acids showed that CsMRP4 transports bile acids through the inner cavity. Moreover, it was found that CsMRP4 mRNA was more abundant in the metacercariae than in the adults. Mouse immune serum, generated against the CsMRP4-NBD1 (24.9 kDa) fragment, localized CsMRP4 mainly in mesenchymal tissues and oral and ventral suckers of the metacercariae and the adults. Our findings shed new light on MRPs and their homologs and provide a platform for further structural and functional investigations on the bile transporters and parasites' survival. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Korea, Republic of | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 33% |
| Student > Master | 2 | 22% |
| Student > Postgraduate | 2 | 22% |
| Professor > Associate Professor | 1 | 11% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 33% |
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Agricultural and Biological Sciences | 1 | 11% |
| Neuroscience | 1 | 11% |
| Medicine and Dentistry | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 1 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 November 2017.
All research outputs
#18,573,839
of 23,005,189 outputs
Outputs from Parasites & Vectors
#4,262
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Outputs of similar age
#325,406
of 437,708 outputs
Outputs of similar age from Parasites & Vectors
#121
of 163 outputs
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